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Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135

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BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135
Textbook Problem

RECALL Why is the use of temperature-stable DNA polymerase an important factor in the polymerase chain reaction?

Interpretation Introduction

Interpretation:

The reason for the fact that the use of temperature-stable DNA polymerase is an important factor in the polymerase chain reaction.

Concept introduction:

Polymerase chain reaction or PCR is the process that is used to make multiple copies of DNA.

PCR is a temperature-based reaction.

Explanation

Polymerase chain reaction or PCR is the process that is used to make multiple copies of DNA. PCR is a temperature-based reaction where all the three steps—denaturation, annealing and tagging of Taq polymerase—require different temperature conditions.

In the first step, DNA strands are separated or denatured at 95°C. Then these DNA strands are cooled down to 55°C in order to start annealing in which primers or oligonucleotides are tagged with strands to synthesize complementary strand

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