Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 13, Problem 35RE
RECALL What are the criteria for “good” primers in a PCR reaction?
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Recall the semi discontinuity in DNA replication. Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semidiscontinuous in in vitro replication? PLEASE EXPLAIN COMPLETELY AND NOT JUST COPY PASTE ANSWERS FROM GOOGLE.
Chapter 13 Solutions
Biochemistry
Ch. 13 - RECALL What advantages does fluorescent labeling...Ch. 13 - RECALL What methods are used to visualize...Ch. 13 - REFLECT AND APPLY When proteins are separated...Ch. 13 - RECALL How does the use of restriction...Ch. 13 - RECALL What is the importance of methylation in...Ch. 13 - RECALL Why do restriction endonucleases not...Ch. 13 - Prob. 7RECh. 13 - Prob. 8RECh. 13 - RECALL What do the following have in common? MOM;...Ch. 13 - RECALL Give three examples of DNA palindromes.
Ch. 13 - RECALL What are three differences between the...Ch. 13 - RECALL What are sticky ends? What is their...Ch. 13 - RECALL What would be an advantage of using HaeIII...Ch. 13 - RECALL Describe the cloning of DNA.Ch. 13 - RECALL What vectors can be used for cloning?Ch. 13 - RECALL Describe the method you would use to test...Ch. 13 - RECALL What is blue/white screening? What is the...Ch. 13 - Prob. 18RECh. 13 - Prob. 19RECh. 13 - Prob. 20RECh. 13 - Prob. 21RECh. 13 - Prob. 22RECh. 13 - Prob. 23RECh. 13 - REFLECT AND APPLY What are the requirements for an...Ch. 13 - Prob. 25RECh. 13 - Prob. 26RECh. 13 - REFLECT AND APPLY The genes for both the a- and...Ch. 13 - REFLECT AND APPLY Outline the methods you would...Ch. 13 - Prob. 29RECh. 13 - Prob. 30RECh. 13 - Prob. 31RECh. 13 - Prob. 32RECh. 13 - RECALL Why is temperature control so important in...Ch. 13 - RECALL Why is the use of temperature-stable DNA...Ch. 13 - RECALL What are the criteria for good primers in a...Ch. 13 - REFLECT AND APPLY What difficulties arise in the...Ch. 13 - REFLECT AND APPLY Each of the following pairs of...Ch. 13 - RECALL What is qPCR?Ch. 13 - Prob. 39RECh. 13 - REFLECT AND APPLY Suppose that you are a...Ch. 13 - REFLECT AND APPLY Why is DNA evidence more useful...Ch. 13 - REFLECT AND APPLY Give the DNA sequence for the...Ch. 13 - Prob. 43RECh. 13 - Prob. 44RECh. 13 - Prob. 45RECh. 13 - Prob. 46RECh. 13 - Prob. 47RECh. 13 - RECALL Has proteomic analysis been done on...Ch. 13 - Prob. 49RECh. 13 - Prob. 50RECh. 13 - Prob. 51RECh. 13 - RECALL What are the key differences between DNA...
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- RECALL What would be an advantage of using HaeIII for a cloning experiment? What would be a disadvantage?arrow_forwardREFLECT AND APPLY Why is it more important for DNA to be replicated accurately than transcribed accurately?arrow_forwardRECALL Give the sequence on the opposite strand for ACGTAT, AGATCT, and ATGGTA (all read 53).arrow_forward
- REFLECT AND APPLY Give the DNA sequence for the template strand that gives rise to the following sequence gel, prepared using the Sanger method with a radioactive label at the 5' end of the primer.arrow_forwardRECALL Why is temperature control so important in the polymerase chain reaction?arrow_forwardREFLECT AND APPLY What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?arrow_forward
- RECALL What are sticky ends? What is their importance in recombinant DNA technology?arrow_forwardREFLECT AND APPLY Suggest a reason why it would be unlikely for replication to take place without unwinding the DNA helix.arrow_forwardRECALL What is blue/white screening? What is the key feature of a plasmid that is used for it?arrow_forward
- REFLECT AND APPLY In the MeselsonStahl experiment that established the semiconservative nature of DNA replication, the extraction method produced short fragments of DNA. What sort of results might have been obtained with longer pieces of DNA?arrow_forwardREFLECT AND APPLY Is the following statement true or false? Why? The flow of genetic information in the cell is always DNARNAprotein.arrow_forwardRECALL What are the key differences between DNA microarrays and protein microarrays, and how they are used in research?arrow_forward
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