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Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135

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BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135
Textbook Problem

REFLECT AND APPLY What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?

Interpretation Introduction

Interpretation:

The difficulties that arise in a polymerase chain reaction if there is contamination of the DNA that is to be copied are to be discussed.

Concept introduction:

Polymerase chain reaction or PCR is the process that is used to make multiple copies of DNA.

PCR is a temperature-based reaction.

Explanation

Polymerase chain reaction or PCR is the process that is used to make multiple copies of DNA. PCR is a temperature-based reaction where all the three steps—denaturation, annealing, and tagging of Taq polymerase—require different temperature conditions.

In the first step, DNA strands are separated or denatured at 95°C

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