Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Chapter 13, Problem 4QP
Summary Introduction
Introduction: The recombinant organism shows the criteria of superiority from the parents. Involvement of genetic sequences takes place in the origination of the hybrid or recombinant organism.
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Which technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro?
a.
The CRISPR-Cas9 system
b.
PCR-based random mutagenesis
c.
PCR-based site-specific mutagenesis
d.
RT-PCR
What do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together?
a.) an amino acid sequence
b.) DNA ligase
c.) restriction enzymes
d.) mRNA
What is the purpose of the low temperature step in the PCR reaction?
a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction
b. To permanently deactivate DNA polymerase
c. To allow primers to anneal to DNA templates
d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' direction
Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- Which process consists of modifying an individual’s genome? a. DNA profiling b. SNPing c. genetic engineering d. DNA hybridization e. PCRarrow_forwardWhich one is an example of nucleic acid hybridization? A the template and newly synthesized DNA strands in replication B reverse transcription of a human RNA into cDNA C association of two strands, one synthetic, by complementarity D synthesis of copy strands by PCR E gene cloning in bacteriaarrow_forwardWhat is the role of Proteinase K in DNA isolation? A. Inhibit enzymes B Catalyze breakdown of nuclei acids C Degrade proteins D Remove disultide bongs in proteinsarrow_forward
- Why must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forwardChoose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forward_____ can fix a genetic flaw in a human. a. Cloning b. Electrophoresis c. Sequencing d. Gene therapyarrow_forward
- Write a short essay that explains how recombinant DNA techniques were used to identify and study genes compared to how modern genomic techniques have revolutionized the cloning and analysis of genes.arrow_forwardseparates molecules by movement due to size and electrical charge [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d. restriction enzymesarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? A. Enzymes unwind and separate the two strands of DNA. B. Repair enzymes correct potential mutations in the DNA sequence. C. Primers base-pair with the exposed single DNA strands. D. DNA ligase seals any gaps remaining between bases of new DNA.arrow_forward
- Which step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forwardWhich technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardBased on what you already know, explain two reasons why is it important for scientists to beable to remove DNA from an organism? Keep in mind that an organism includes any livingthing, from bacteria to humans.arrow_forward
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