Textbook Problem

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Cloning Genes Is a Multistep Process

Restriction enzymes:

1. a. recognize specific nucleotide sequences in DNA
2. b. cut both strands of DNA
3. c. often produce single-stranded tails
4. d. do all of these
5. e. do none of these

Summary Introduction

Introduction: A restriction enzyme is a form of a protein that is produced by bacteria. These enzymes also termed as “restriction endonucleases.” The restriction enzymes play an important role in the production of recombinant DNA.

Explanation of Solution

Reason for correct answer:

Restriction enzymes are capable of forming incisions at the specific positions by reading the particular sequence of nucleotide. The enzyme hydrolyzes the hydrogen bonds between the various nucleotides and helps in the formation of single-stranded DNA. These enzymes are produced by the functioning of the bacterial cell and are able to produce single-stranded tails.

Option d. is given as “do all of these.”

Restriction enzymes are responsible for the “formation of cuts on the DNA strands at specific sites by recognizing particular nucleotide sequences. It works on both the strands of DNA and also produces single-stranded tails.” Hence, the correct answer is option d.

Reason for incorrect answer:

Option a. is given as, “recognize specific nucleotide sequences in DNA.”

Restriction enzymes also form the cuts in the strand of DNA...