Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 13, Problem 6P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Using Graphical Methods to Derive the Kinetic Constants for an Ordered, Single-Displacement Reaction The general rate equation for an ordered, single-displacement reaction where A is the leading substrate is

v = V max [ A ] [ B ] ( K s A K m B + K m A [ B ] + K m B [ A ] + [ A ] [ B ] ) Write the Lineweaver-Burk (double-reciprocal) equivalent of this equation and from it calculate algebraic expressions for the following:

a. The slope

b. The y-intercepts

c. The horizontal and vertical coordinates of the point of intersection when 1/v is plotted versus 1/[B] at various fixed concentrations of A

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