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Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135

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BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
ISBN: 9781305961135
Textbook Problem

RECALL Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?

Interpretation Introduction

Interpretation:

The reason for the statement “restriction endonuclease does not hydrolyze DNA from the organism that produces” is to be determined.

Concept introduction:

DNA fragments are separated by using gel electrophoresis.

DNA is fragmented by using restriction endonucleases.

Explanation

DNA fragments are separated by using gel electrophoresis. In gel electrophoresis, DNA molecules are first fragmented by using restriction endonuclease, which cuts the fragments at specific sites. Methylation entails the addition of the methyl group. When the methyl group is added to a specific site, it prevents that site from cleavage...

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