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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 13, Problem 9QP
Textbook Problem
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Cloning Genes Is a Multistep Process

Assume restriction-enzyme sites as follows: E = EcoRI, H = HindIII, and P = PstI. What size bands (in kilobases) would be present when the DNA shown below is cut with:

  1. a. EcoRI
  2. b. HindIII and PstI
  3. c. all three enzymes

Chapter 13, Problem 9QP, Cloning Genes Is a Multistep Process Assume restriction-enzyme sites as follows: E = EcoRI, H =

a.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with EcoRI.

Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

Explanation of Solution

The restriction enzymes are responsible for the formation of incisions in the DNA strands at a specific position by recognizing the particular nucleotide sequence. Different bands obtained when DNA is cut with EcoRI will be: 2 kb (the first band), 11...

b.

Summary Introduction

To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

c.

Summary Introduction

To explain: The size of bands obtained after the cutting of DNA with all three enzymes.

Introduction:

Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.

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