Concept explainers
a.
To determine: The size of bands obtained after the cutting of DNA with EcoRI.
Introduction: Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
b.
To determine: The size of bands obtained after the cutting of DNA with HindIII and PstI.
Introduction:
Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
c.
To explain: The size of bands obtained after the cutting of DNA with all three enzymes.
Introduction:
Every restriction enzyme is specific in forming the incisions in the strand of DNA by recognizing the specific sites in genome sequence. The DNA strands are formed from the joining of various nucleotides that are linked to each other by hydrogen bonding. Nucleotides are attached to each other by being complementary in origin.
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Human Heredity: Principles and Issues (MindTap Course List)
- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardDuring an Ames test, what does it mean when S. typhimurium is his+? A. The sample is completely safe for humans B. The sample is safe for humans but has a chance to be a mutagen C. The sample is not safe for humans and is considered a mutagen D. The sample is not safe and is hypothetically a mutagenarrow_forward
- You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a. Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above,arrow_forwardCloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5arrow_forwardFor each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate the same gene from 20 individuals.b. Isolate 100 genes from the same individual.c. Isolate a mouse gene when you have a rat genefragment.arrow_forward
- In next-generation sequencing, which of these advances allows for massively parallel sequencing? a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece. b. DNA sequences are read in real-time as nucleotides are added to each piece. c. Each segment of the genome can be pieced back together through shotgun alignment d. Single molecules of DNA can be read without the need for amplification.arrow_forwardExplain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait an important part of the recombinant DNA technology process in the biotechnology industry?arrow_forwardWhich of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.arrow_forward
- The first publications describing the successful production and intracellular replication of recombinant DNA appeared in the early 1970’s. Since then, there is a long list of breakthroughs achieved by recombinant DNA technology. But which one of the following are NOT on that list? Select more than one answer ifneeded. a) Recombinant Factor VIII protein for the treatment of hemophiliacs b) Positional cloning of over 100 human disease genes c) Bacteria producing human insulin for the treatment of diabetic patients d) Reproductive cloning of over a dozen humansarrow_forwardIf you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardIn genetic engineering, can we insert the gene from human to E. coli (bacteria)? Explain your answer.arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning