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DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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Section
BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 14, Problem 12QP
Textbook Problem
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DNA Profiles as Tools for Identification

A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?

Chapter 14, Problem 12QP, DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that

Summary Introduction

To determine: The male(s) which can be excluded as the father of the child after examining the results of the Southern Blot in reference to the textbook.

Introduction: The genetic material of an individual is packed in the form of DNA. Shorter nucleotide sequences known as short tandem repeats or STRs are used by law enforcement agencies to develop DNA profiles. A unique DNA fragment pattern is produced by STRs from a single chromosome. Criminal cases and other cases such as paternity cases use DNA profiles.

Explanation of Solution

The observations made on the basis of the given southern blot are as follows:

  • • Two bands of DNA are observed. One band is 10 kb long and another band is 5 kb long.
  • • The 10 kb long DNA amplicon is assumed to be inherited fro...
Summary Introduction

To determine: The male(s) which could be the father of the child.

Introduction: The genetic material of an individual is packed in the form of DNA. Shorter nucleotide sequences known as short tandem repeats or STRs are used by law enforcement agencies to develop DNA profiles. A unique DNA fragment pattern is produced by STRs from a single chromosome. Criminal cases and other cases such as paternity cases use DNA profiles.

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