Chapter 14, Problem 12TYK

Summary Introduction

To review:

The effects of exposing eukaryotic cells to radioactive thymidine during the S or synthesis phase of interphase, on sister chromatids of the duplicated chromosome at metaphase of the mitosis, after the second round of DNA (deoxyribonucleic acid) replication in a medium free of radioactive labels.

Introduction:

DNA of eukaryotic chromosomes replicate semi-conservatively. This was illustrated by labeling the cells with radioactive thymidine during the S-phase and then grown in the absence of radioactive thymidine. The cell cycle was then studied. The cell cycle consists of interphase and mitotic phase. DNA is synthesized during the S-phase of interphase whereas the mitotic phase is the divisional phase and consists of prophase, metaphase, anaphase, and telophase.