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DNA Profiles as Tools for Identification A crime is committed, and the only piece of evidence the police are able to gather is a small bloodstain. The forensic scientist at the crime lab is able to: extract DNA from the blood, cut the DNA with a restriction enzyme, separate the fragments by electrophoresis, and transfer the DNA from a gel to a membrane and probe with radioactive DNA. Probe 1 is used to visualize the pattern of bands. The forensic scientist compares the band pattern in the evidence (E) with the patterns from the suspects (S1, S2). The first probe is removed, the membrane is hybridized using another probe (Probe 2), and the band patterns are compared. This process is repeated for Probe 3 and Probe 4. a. Based on the results of this testing, can either of the suspects be excluded as the one who committed the crime? b. If so, which one? Why? c. Is the pattern from the evidence consistent with the band pattern of one of the suspects? Which one?

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 14, Problem 9QP
Textbook Problem
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DNA Profiles as Tools for Identification

A crime is committed, and the only piece of evidence the police are able to gather is a small bloodstain. The forensic scientist at the crime lab is able to:

  • extract DNA from the blood,
  • cut the DNA with a restriction enzyme,
  • separate the fragments by electrophoresis, and
  • transfer the DNA from a gel to a membrane and probe with radioactive DNA.

Probe 1 is used to visualize the pattern of bands. The forensic scientist compares the band pattern in the evidence (E) with the patterns from the suspects (S1, S2). The first probe is removed, the membrane is hybridized using another probe (Probe 2), and the band patterns are compared. This process is repeated for Probe 3 and Probe 4.

  1. a. Based on the results of this testing, can either of the suspects be excluded as the one who committed the crime?
  2. b. If so, which one? Why?
  3. c. Is the pattern from the evidence consistent with the band pattern of one of the suspects? Which one?

Chapter 14, Problem 9QP, DNA Profiles as Tools for Identification A crime is committed, and the only piece of evidence the

a.

Summary Introduction

To determine: Whether, any of the suspects can be excluded as the one who committed the crime based on the results of the testing, or not.

Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.

Explanation of Solution

The radioactive DNA is used to identify the criminal as the sequence of this DNA is complementary to the sequence of the DNA found in the evidence. The DNA molecules are traced with the help of radioactivity of the radioactive DNA probes...

b.

Summary Introduction

To determine: The suspect which can be excluded as the one who committed the crime with a plausible reason.

Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.

c.

Summary Introduction

To determine: Whether the pattern from the evidence is consistent with the band pattern of one of the suspects, or not and also determine the suspect with which it is consistent.

Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.

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