Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 14.4, Problem 1TC
For any single person, a given STR always has either one or two bands. Why? Further, single bands are always about twice as bright as each band of a pair. For example, in the D16 STR on the right, the single bands of the first and third DNA samples are twice as bright as the pairs of bands of the second, fourth, and fifth samples. Why?
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Chapter 14 Solutions
Biology
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.1 - define GMO and transgenic organism?Ch. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Prob. 1CSCCh. 14.3 - Prob. 1CYLCh. 14.3 - summarize how CRISPR-Cas9 works and explain why it...Ch. 14.4 - For any single person, a given STR always has...Ch. 14.4 - There are many other applications in which DNA...Ch. 14.4 - Prob. 1CYL
Ch. 14.4 - Prob. 2CYLCh. 14.5 - Restriction enzymes are isolated from bacteria....Ch. 14.5 - Many countries regulate the use of genetically...Ch. 14.5 - explain how genes are inserted into a plasmid, and...Ch. 14.5 - Prob. 2CYLCh. 14.6 - Prob. 1CTCh. 14.6 - Prob. 1HYEWCh. 14.6 - describe the advantages of genetically modified...Ch. 14.6 - list some examples of how genetically modified...Ch. 14.6 - Prob. 3CYLCh. 14.7 - Explain how fetal DNA could be used to establish...Ch. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe how transgenic organisms are used to...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - Prob. 2CYLCh. 14.8 - Prob. 1CTCh. 14 - Prob. 1MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Describe two natural forms of genetic...Ch. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - Prob. 6RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- How would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7% agarose gel slab? For separating small fragments of DNA, is it better to use a 7% gel or a 0.1% gel? Why?arrow_forwardIn figure 10.16 , how would the curve appear if the GC content of the DNA sample were increased? How would the curve appear if the AT content were increased?arrow_forwardWhy is it important that the alcohol used in the DNA extraction is kept cold?arrow_forward
- Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?arrow_forwardCan you please interpret and discuss and mark, this gel electrophoresis results. DNA ladder sizes: Band1: 23130, Band2: 9416, Band3: 6557, Band4: 4361, Band5: 2322, Band6: 564, Band7: 125arrow_forwardEven in automated sequencing, where you can include all 4 ddNTPs in one reaction, you need to include "normal" dNTPs as well as the ddNTPs - why are these necessary? Why can't you just put in the ddNTPs, since you've got all four of them available to the DNA polymerase?arrow_forward
- In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?arrow_forwardHow would you estimate the size of the unknown DNA fragement just by looking at the gel?arrow_forwardIn typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is not the source of that sample,whereas a DNA match does not necessarily prove that a suspect is the source?arrow_forward
- In our simple model of DNA amplification we assumed that a successful amplification doubled the DNA each cycle. Reality isn't so kind! Two related problems encountered in DNA profiling are the issues of asymmetric peaks and "drop-out" where an allele produces a small to very-small peak compared to the other allele. Assume you have a sample with a heterozygous set of alleles. Let one of the alleles be successfully doubled each cycle but the second allele is (on average) only increased by 1.8. If the sample is subjected to 30 PCR cycles, how much larger will the "successful" peak be than the "less successful" peak? Set your answer up as a ratio of the "successful" /"unsuccessful" and round your number to 1 decimal place. (for example, if your calculations show that after 30 cycles the successful allele has 55340 copies and the unsuccessful has 10000 copies the ratio is 5.5)arrow_forwardDo you think DNA should be collected and genetic information entered into CODIS under any circumstances? If yes, do you think DNA should be collected from persons who are merely arrested or should a person be convicted of a crime before DNA can be collected? Would any crime qualify for DNA collection or just certain crimes? Which crimes? How long should CODIS retain that information? Who should be able to access that information and under what circumstances?arrow_forwardThe region of the normal hemoglobin gene used for genetic testing for sickle cell anemia contains a restriction site such that homozygous normal individuals show two DNA fragments. If a single nucleotide change in hemoglobin destroys that restriction site, then how many DNA fragments will be visible on a gel from individuals that are homozygous mutant? What about heterozygotes?arrow_forward
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