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Biology (MindTap Course List)

11th Edition
Eldra Solomon + 3 others
Publisher: Cengage Learning
ISBN: 9781337392938

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BuyFindarrow_forward

Biology (MindTap Course List)

11th Edition
Eldra Solomon + 3 others
Publisher: Cengage Learning
ISBN: 9781337392938
Chapter 15, Problem 12TYU
Textbook Problem
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What are some of the problems that might arise if you were trying to produce a eukaryotic protein in a bacterium? How might using transgenic plants or animals help solve some of these problems?

Summary Introduction

To determine: The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems.

Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.

Explanation of Solution

The cloning of the eukaryotic gene in a prokaryotic bacterial plasmid involves many problems since eukaryotic DNA consists of introns, and prokaryotes do not have RNA-splicing enzymes and machinery to carry out RNA processing. Bacterial plasmids are unable to process pre-mRNA to mature RNAs. Post-transcriptional processing is necessary for normal functioning of eukaryotic proteins. During RNA processing, the pre-mRNA synthesized is processed to remove non-coding sequences (introns) and form mature mRNA...

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Chapter 15 Solutions

Biology (MindTap Course List)
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Ch. 15.2 - Explain how modified forms of CRISPR/Cas...Ch. 15.2 - VISUALIZE Draw a diagram illustrating a bacterial...Ch. 15.2 - Describe how a gene might be deleted in a zygote...Ch. 15.2 - Describe how a point mutation in a eukaryotic gene...Ch. 15.3 - Contrast the early DNA sequencing methods with...Ch. 15.3 - Describe how gene databases are used to gain...Ch. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Explain how you might use GenBank to obtain...Ch. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - PREDICT Exposing C. elegans to an RNAi molecule...Ch. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - In what way has the production of human insulin by...Ch. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Explain how a CRISPR endonuclease system can be...Ch. 15.6 - Explain why a gene drive locus can spread through...Ch. 15.6 - How does a gene drive spread through a population?Ch. 15.6 - What is the difference between a gene drive and a...Ch. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Human DNA and particular plasmid both have sites...Ch. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - The PCR technique uses (a) heart-resistant DNA...Ch. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - The dideoxynucleosides ddATP, ddTTP, and ddCTP...Ch. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - What are some of the problems that might arise if...Ch. 15 - CONNECT Would genetic engineering be possible if...Ch. 15 - INTERPRET DATA STR analysis was performed on blood...Ch. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...

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