Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 15, Problem 15.13QAP
Interpretation Introduction
Interpretation:
The part of the molecule that behaves as the chromophore and fluorescent centre should be determined.
Concept introduction:
The chemical compound obtained from the bark of the cinchona tree is called Quinine. The molecule that is most likely to behave as the chromophore and fluorescent centre.Chromophore is a group of molecule or chromophore is an atom which shows the colour of a compound.
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A molecule phosphoresces with a single peak wavelength of 700 nm. The single fluorescence peak is at 590 nm. The absorption peak is at 490 nm. Based on this information, roughly sketch the Jablonski diagram, labeling transitions and calculating differences in each energy state in nm and cm-1.
Only typed solution.
Using the light scattering technique, an example of a nonideal interfering condition for a high-mass biomolecule sample analysis could relate to which one of these?
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B) Selecting samples from which the identity has been masked. This procedure prevents the analyst from prejudging the outcome of the analysis.
C) Removing any material that may interfere with an analysis.
Chapter 15 Solutions
Principles of Instrumental Analysis
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