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Biology: The Unity and Diversity o...

15th Edition
Cecie Starr + 3 others
Publisher: Cengage Learning
ISBN: 9781337408332

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Chapter
Section
BuyFindarrow_forward

Biology: The Unity and Diversity o...

15th Edition
Cecie Starr + 3 others
Publisher: Cengage Learning
ISBN: 9781337408332
Chapter 15, Problem 6SQ
Textbook Problem
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_______ is the technique of determining the order of nucleotide bases in a sample of DNA.

  1. a. PCR
  2. b. Sequencing
  3. c. Electrophoresis
  4. d. Nucleic acid hybridization

Summary Introduction

Introduction: Deoxyribonucleic acid (DNA) is a macromolecule that is a polymeric chain of nucleotides. Every nucleotide is made up of a sugar base, a phosphate group, and any one of the four nitrogenous bases (adenine (A), thymine (T), guanine (G), and cytosine (C). The DNA carries the genetic information of a cell, which is determined by the sequence of arrangement of nucleotides in the DNA strand.

Explanation of Solution

Reason for correct answer:

The DNA carries the genetic information of a cell, which is determined by the order of nucleotides arranged in the DNA strand. To know the order of nucleotides in DNA, several techniques are applied, which are called as DNA sequencing. One of the sequencing methods is based on DNA replication and uses labeled nucleotides and electrophoresis to find the nucleotide sequence of a given DNA sample.

Hence, the correct answer is option b.

Reason for incorrect answer:

Option a. is given as “PCR”.

Polymerase chain reaction (PCR) is a technique that is used to create multiple copies of a DNA segment by repeated cycles of replicating it using primers and heat-resistant DNA polymerase enzymes. Thus, PCR is used to amplify a DNA sequence and not to find its nucleotide sequence...

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