BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 16, Problem 1DA
Summary Introduction

To determine: The phenotype of a repressor mutation which prevents DNA binding.

Introduction: A repressor molecule or protein prevents the RNA polymerase from binding to the promoter region and thus, prevents the expression of genes.

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Explanation of Solution

The phenotype of a repressor mutation in which binding of DNA is prevented would be the expression of the genes which facilitate lactose metabolism. This is because the operator would not be able to bind to the repressor and transcription would get inhibited.

Summary Introduction

To determine: The phenotype of a repressor mutation that prevents DNA binding and an operator mutation that prevents repressor binding.

Introduction: In molecular biology, operator is a genetic sequence which is responsible for facilitating the transcribing proteins to bind to the DNA sequence being transcribed.

Expert Solution
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Explanation of Solution

Mutation in the operator or inducer that prevents the binding of the repressor would allow the operator to bing to the DNA all the time and the genes for lactose metabolism would not get expressed. This is because the RNA polymerase would bind to the DNA and transcription would proceed. This is in contrast to the phenotype expressed when mutation in the repressor would prevent transcription.

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Students have asked these similar questions
What detection method could be used to determine the Mutation of the operator region that binds a repressor in a gene locus and what results could be achieved from that?
What role does an operator sequence serve in bacterial gene expression regulation? Describe one change in bacterial cells that can cause a repressor protein to go from inactive to active or from active to inactive.
What would happen to the regulation of the tryptophan operon in bacterial cells that express a mutant form of the tryptophan repressor that (1) cannot bind to DNA, (2) cannot bind tryptophan, or (3) binds to DNA even in the absence of tryptophan?

Chapter 16 Solutions

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY