Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 14TYK
Summary Introduction
To review:
The experiment that can confirm the presence of a wild type bio+ allele in the plasmid of chromosomal DNA (deoxyribonucleic acid).
Introduction:
Minimal media is the media that only provides minimum requirement that is only inorganic salts and no amino acids. The colonies whose genome is complete, that is, they have genes for all requirements grows on the minimal media while others do not.
Electroporation is the technique in which electric pulse is given to the culture leading the opening of the transient pores in the cells that can allow the entry of the DNA from the surrounding.
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Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardFigure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.arrow_forwardOne experiment that showed the DNA carried genetic information was the "Transformation" experiment by Avery, McCarty, and MacLeod where they injected mice with non-pathogenetic bacteria that had been incubated in the remains of pathogenic bacteria. What did they find when they incubated non-pathogenic bacteria in just the protein from the pathogenic bacteria? In this case there was no transformation They found that the non-pathogenic bacteria were transformed into pathogenic bacteria In this case the mice were sickened but did not die They found that some of the protein could cause transformation but other proteins could notarrow_forward
- If you were doing a cloning experiment where a plasmid was treated with PstI but the insert DNA did not have a PstI restriction site, what restriction enzyme(s) would be suitable to treat the DNA to be inserted?arrow_forwardA genetics instructor designs a laboratory experiment to study the effects of UV radiation on mutation in bacteria. In the experiment, the students spread bacteria on petri plates, expose the plates to UV light for different lengths of time, place the plates in an incubator for 48 hours, and then count the number of colonies that appear on each plate. The bacteria that have received more UV radiation should have more pyrimidine dimers, which block replication; thus, fewer colonies should appear on the plates exposed to UV light for longer periods. Before the students carry out the experiment, the instructor warns them that while the bacteria are in the incubator, the students must not open the incubator door unless the room is darkened. Why should the bacteria not be exposed to light?arrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forward
- You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardWould it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. B. No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. C. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first.arrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forward
- Suppose the experiment of Meselson and Stahl was performed on a sample of 8 cells, each containing one copy of its circular double-stranded DNA genome, and that had been growing on normal 14N medium. You then grew the cells for 3 generations in medium containing 15N. The outcome would be A) 8 cells with single-stranded DNA molecules with 14N, and 24 cells with single-stranded DNA molecules with 15N. B) 16 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 48 cells with double-stranded DNA molecules with 15N. C) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 24 cells with double-stranded DNA molecules with 15N. D) 8 cells with double-stranded DNA molecules with equal amounts of 14N and 15N, and 32 cells with double-stranded DNA molecules with 15N. E) 65 cells with single-stranded DNA molecules with 15N.arrow_forwardWhat would happen in the experiment illustrated in Figure if the DNA and RNA that are mixed together came from very different organisms, for example a worm and a pig?arrow_forwardIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
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