Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 17.4, Problem 1MI
What special considerations are necessary if one is constructing a translational fusion of a protein known to be exported?
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Why chaperones are particularly important when translation occurs on membrane-bound ribosomes ?
what is post-translational protein covalent modification? give three examples and name the enzyme that catalyzes each reaction.
A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-ProIle-Ser-Ala. What would be the effect on translation if the following component were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning.
Q. Initiation factor 3
Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- How do siRNAs and miRNAs target specific mRNAs for degradation or for the repression of translation?arrow_forwardPlease describe the four-step process of the elongation during protein translation in bacteria.arrow_forwardA synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-ProIle-Ser-Ala. What would be the effect on translation if the following component were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. Q.Release factors RF-1, RF-2, and RF-3arrow_forward
- For each of the following initiation factors, how would eukaryoticinitiation of translation be affected if it were missing?A. eIF2B. eIF4C. eIF5arrow_forwardWhat are the two most common methods of post translation modification of proteins?arrow_forwardTranslational control is usually aimed at preventing the initiation of translation. With regard to cellular efficiency, why do you think this is the case?arrow_forward
- All translation begin at free ribosomes in the cytosol, yet all secreted proteins are translocated into the ER. What is the one molecule that is responsible for targeting co-translationally translocated proteins to the ER and what are the three events that this molecule mediates between translational initiation and insertion into the ER?arrow_forwardDescribe the various post-transcriptional and post-translational modifications that occur during the transition from pre-mRNA to mature protein.arrow_forwardA synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-Pro- Ile-Ser-Ala. What would be the effect on translation if the following components were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. a. Initiation factor 3 b. Initiation factor 2 c. Elongation factor Tu d. Elongation factor G e. Release factors RF-1, RF-2, and RF-3 f. ATP g. GTParrow_forward
- Do the following events during bacterial translation occur primarily within the 30S subunit, within the 50S subunit, or at the interface between these two ribosomal subunits? A. mRNA-tRNA recognition B. Peptidyl transfer reaction C. Exit of the polypeptide from the ribosome D. Binding of initiation factors IF1, IF2, and IF3arrow_forwardWhat is the peptide encoded by this mRNA sequence 5’-UCU-GCA- AAU-UAA -GUU-3’?arrow_forwardWhat is the "genetic code" and what aspect of post translational modification virtually mandates that "genetic code" applies to mRNA rather than DNA?arrow_forward
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