Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 18, Problem 10TYK
Summary Introduction

Introduction:

DNA fingerprinting is a method which is used to identify the DNA (deoxyribonucleic acid) of different species based on the occurrence of variable number tandem repeats (VNTR). It is done through restriction digestion of nucleotide sequences and the separation of these sequences in electrophoresis. Then, it is tagged with DNA probe to visualize the different patterns of DNA sequences for its identification and comparison.

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In next-generation sequencing, which of these advances allows for massively parallel sequencing?       a.  Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece.       b.  DNA sequences are read in real-time as nucleotides are added to each piece.       c.  Each segment of the genome can be pieced back together through shotgun alignment       d.  Single molecules of DNA can be read without the need for amplification.
A person cannot see a single cotton thread 100 feet away, but if you wound thousands of threads together into a rope, it would be visible much further away. Is this statement analogous to our DNA extraction? Explain.
DNA sequencing technology has been around since the late 1970s. Why didsequencing whole genomes present a challenge?
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