2 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781264439218
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 18, Problem 2U
Summary Introduction
Introduction:
Restriction mapping is technically easy, high throughput, high resolution and technically easy mapping of small DNA molecules, on the other hand FISH allow low resolution mapping of the enormous DNA molecule. STS is Sequence tagged site mapping that emerge as a powerful alternative which combines the best of mapping through FISH and best of restriction mapping.
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a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?
What is a genomic DNA library?
O A. All DNA fragments identified with a probe
O B. A general collection of all genes sequenced thus far
C. A DNA fragment inserted into a vector
O D. A collection of DNA fragments that make up the entire genome of a particular organism that have been cloned into a vector
O E. A collection of DNA fragments that make up the entire genome of a particular organism
Short tandem repeats (STR) profiling is based on
O A. the fact that many foods are being genetically modified and this test allows food health officials to identify the transgenic ones.
O B. the fact that you can clone mammals through fusion of one somatic (non-sex) cell with an egg cell whose nucleus has been removed
O C. the fact that one strand of DNA can be turned into millions of identical copies by a process that heats and cools DNA and builds it using DNA polymerase and primers.
OD.
the fact that people's DNA is filled with short sequences, like "TCAT" that are found in different numbers in each person.
Chapter 18 Solutions
2 SEM ACC W/RAVEN CARDED
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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- a. What sequence information about a gene is lackingin a cDNA library?b. Can clones in a cDNA library contain 5′ UTR sequences? 3′ UTR sequences?c. Would you be likely to find on average longerORFs in cloned sequences from a genomic libraryor from a cDNA library? Explainarrow_forwarda. The reading frame DNA sequence is b. The mRNA sequence is c. The polypeptide sequence is a.The mutated polypeptide sequence is b.What kind of mutation was produced?arrow_forwardWhat Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.arrow_forward
- Match each of the terms in the left column to the bestfitting phrase from the right column.a. exome 1. a discrete part of a protein that provides a unitof functionb. de novo gene 2. a nonfunctional member of a gene familyc. gene desert 3. the joining together of exons in a gene indifferent combinationsd. pseudogene 4. most frequent residues, either nucleotide oramino acid, found at each position in asequence alignmente. syntenic block 5. set of genes related by processes ofduplication and divergencef. orthologs 6. chromosomal region with the same genes inthe same order in two different speciesg. naturalselection7. genes with sequence similarities in twodifferent species that arose from a commonancestral geneh. consensussequence8. genes that arose by duplication within aspeciesi. gene family 9. genomic DNA sequences containing exonsj. paralogs 10. gene-poor region of the genomek. alternativeRNA splicing11. recently evolved from intergenic DNAsequencesl. protein domain 12. progressive…arrow_forwardAn organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. 0.000 de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genomearrow_forwardQ. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.arrow_forward
- A. What are some reasons a person might want to clone a human? B. What animal was cloned in 1885?arrow_forwardWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forwarda. The reading frame DNA sequence is: b. The mRNA sequence is: c. The polypeptide sequence is: A disease in frogs which causes their tongue to fall out of their mouths is killing the frog population in LA County. You obtain a dead frog and isolate its gene Xf. When you sequence this mutated gene, you find that the last ‘G’ at the end of the first line of this sequence has been deleted (i.e. the G at position 86). In order to determine how this mutation changes the resulting polypeptide, write the mutated polypeptide sequence in the space below. What kind of mutation was produced? The mutated polypeptide sequence is What kind of mutation was produced?arrow_forward
- In Figure 10-10, determine approximately how manyBAC clones are needed to provide 1× coverage ofa. the yeast genome (12 Mbp).b. the E. coli genome (4.1 Mbp).c. the fruit-fly genome (130 Mbp).arrow_forwardH2. Please explain with details also explain wrong optionsarrow_forwarda. What type of nucleic acid and from what species would the scientist use to begin construction of her genomic DNA library? b. From what tissue would she isolate this nucleic acid? c. What type of reagent would the scientist use to cut the genome into appropriately sized fragments? d. What size nucleic acid fragments would one aim to prepare for the library construction so as to to avoid having to screen an overwhelming number of clones? e. Into what vector would the scientist ligate her genomic DNA fragments? f. What organism would the scientist use to propagate the clones of her genomic DNA library? g. From the information given in the problem determine what probe could be used to screen the scientist's library to find her clone of interest ?arrow_forward
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