2 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781264439218
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 18, Problem 8U
Summary Introduction
Introduction:
Proteome is the collection of all proteins present in a cell and the set of “all RNA molecules” present in cell is known as transcriptome. Transcriptome can vary with environmental conditions because it is not fixed like genome. It also reflects the genes that are actively expressed at a particular time.
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A mouse gene was identified and determined to be required for formation of heart muscle. A gene with a similar sequence was identified in the human genome. What experiment could scientists do to determine if the mouse and human genes have similar functions?
A. The scientist could place the normal human gene into normal mice and see if the resulting mice are viable.
B. The scientist could search the human genome for genes that encode proteins that are identical to the protein encoded by the mouse gene.
C. The scientist could place the normal human gene into mutant mice to see if heart muscle forms in the mouse.
D. The scientist could place the mutant mouse gene into humans to see if humans develop without heart muscle.
1a) Why is it possible for you to study the eye colour gene by extracting cheek cells?
a. Because the nucleus of every cell in the human body contains the same genetic information.
b. Because the cheek cells are located near the cells of the eye and so they are able to exchange DNA.
c. Because all genes in the human body are expressed at all times so it is easy to study them.
d. All of the above are possible explanations.
1b) What is the purpose of heating the sample to 75°C following addition of the 0.2M NaOH solution?
a. To denature the histone proteins that are keeping the DNA tightly coiled.
b. To ensure that all the DNA is removed from the swab in preparation for PCR.
c. To breakdown the cheek cell membrane to release the DNA from the cell.
d. It breaks down the circular DNA down into linear fragments so that they will be easier to visualize.i
Which statement BEST differentiate metagenomics from proteomics?
a The material used in metagenomics is mRNA while proteomic uses polypeptides.
b The material used in metagenomics is DNA while proteomic uses polypeptides.
c The material used in metagenomics is a transcriptome while proteomic uses polypeptides.
d The material used in metagenomics is DNA from a mitochondrion while proteomic uses polypeptides.
Chapter 18 Solutions
2 SEM ACC W/RAVEN CARDED
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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- Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. B. Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons. C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. D. The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.arrow_forwardWhy might a single base-pair mutation in eukaryotic mRNA be less serious than one in prokaryotic mRNA? a. If the mutation occurs in the 5' end of the start site, it will not affect the gene product. b. If the mutation occurs in the exon, it will not affect the gene product. c. If the mutation occurs in the splice site of a transcript with alternative splicing, only one gene product may affected. O d. If the mutation occurs in the intron or not in the splice site of a transcript with alternative splicing, it will nc affect the gene product. O e. If the mutation occurs in the 3' end of the start site, it will not affect the gene product. OLIE STIC N 1Aarrow_forwardIdentify the word or phrase that is the best match (some will remain blank). (Mouse Genome Informatics, GeneChip array, Confounding variable, 45,000, BLAST, 600, Aceview, BioGPS, SMART, minimize, 17, UCSC Genome Browser, External validity) A. Median number of copies of an mRNA in a cell B. Patterns and sizes of introns and exons C. H3K4me3 locations on chromosomes D. Each treatment given to a different litter of mice E. Find list of knockout mice for a gene D. Find similar proteins F. Random variation G. Tissue expression pattern of a gene H. The extent to which results can be extrapolatedarrow_forward
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