Biology: The Dynamic Science (Mind...

4th Edition
Peter J. Russell + 2 others
ISBN: 9781305389892



Biology: The Dynamic Science (Mind...

4th Edition
Peter J. Russell + 2 others
ISBN: 9781305389892
Textbook Problem

Discuss Concepts Why are high-throughput techniques used so much in genomics research? Give examples from Chapter 19 of different uses of high-throughput techniques.

Summary Introduction

To review:

The reason for the use of high-throughput techniques in genomic research and the examples stating different uses of high-throughput techniques.


In order to compare similar genes between species, new throughput techniques are developed in genome sequences in genomic research. The study of genes and deoxyribonucleic acid (DNA) on a large scale can store genomic data sufficiently.


The use of high-throughput technologies in genomic research as a tool for studies, and detailed knowledge of diseases and evolution. It can also be used to study the genes in a genome by DNA (deoxyribonucleic acid) microarray and a complete set of proteins in an organism by mass spectroscopy, X-ray crystallography, and Nuclear Magnetic Resonance (NMR).

There are many high throughput techniques developed for genomic research:

1) DNA microarray is based on hybridization that can be used in the analysis of expression levels. They are mainly used in the study of genes and DNA, also known as DNA chips. The study includes a comparison of the gene expression pattern.

The technique is used to differentiate between two cells and their gene expression by fluorescent labeling. The mixture containing the complementary DNA (cDNA) are fluorescently labeled and are detected when excited by a laser beam. Microarray analysis is used in the analysis of cancer type and sub-type, expression of a normal cell when it is becoming a cancer cell, a genetic disease caused by mutation and gene expression...

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