Interpretation:
The number of molecules of oxaloacetate in the mitochondrion and definition of pOAA should be determined.
Concept Introduction:
The expression used to determine the standard free energy is:
Here
Explanation of Solution
The enzyme which converts malate into oxaloacetate is malate dehydrogenase. The reaction is as shown:
Below reaction is used to determine the standard free energy:
Here
At equilibrium
After rearranging the equation, we will get:
Plugging in the values to determine the oxaloacetate concentration:
Concentration of oxaloacetate is 0.214 µM.
Below formula will be used to determine the volume:
Here, V = Volume, d =diameter, l = length
Plugging the values in the above equation to determine the volume:
Now we have to calculate concentration and volume now determine the oxaloacetate molecules:
There are 20 molecules of oxaloacetate in the mitochondrion.
The pOAA can be determined from the negative log of oxaloacetate concentration:
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Chapter 19 Solutions
Biochemistry