Radiolabeling with 14 C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C y with 14 C.

Question

Want to see more full solutions like this?

Answer 1

Textbook Question

Chapter 19, Problem 1P

Radiolabeling with ¹⁴C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C_y with ¹⁴C.

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

The mitochondrial form of carbamoyl phosphate synthetase is allosterically activated by N-acetylglutamate. Briefly describe a rationale for this effect.

Is trimethoprim a mechanism-based inhibitor of bacterial dihydrofolate reductase?

By analysis of the kinetics of interconversion of (1-13C]FBP and [6-13C]FBP and the appearance of 3-PGA and PEP in further experiments such as shown in the figure above, it was concluded that the rate of oxidation of glyceraldehyde-3-phosphate (GAP) was hindered under conditions of anaerobiosis. Separate experiments showed that the activity of 3-phosphoglcerate kinase was normaland that the concentrations of ADP and ATP were not limiting. What metabolic condition is the most likely to account for this conclusion? Write the reaction using structural formulas and naming reactants

Answer 2

Textbook Question

Chapter 19, Problem 1P

Radiolabeling with ¹⁴C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C_y with ¹⁴C.

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 19, Problem 1P

Radiolabeling with ¹⁴C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C_y with ¹⁴C.

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 19, Problem 1P

Radiolabeling with ¹⁴C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C_y with ¹⁴C.

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Answer 8

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Interpretation Introduction

Interpretation:

The labeling pattern that would result in the introduction of TCA cycle of glutamate Cγ with C14 should be described.

Concept Introduction:

The technique used to track the passage of an isotope or patterns is known as labeling pattern. TCA cycle is abbreviated as tricarboxylic acid. During this cycle, the organism especially aerobic organisms release the energy which will be a stored through the oxidation of acetyl-CoA.

Answer 12

Explanation of Solution

During Mitochondrial Pyruvate Transport, Glutamate maintains the TCA cycle and survival of the cell. The labeling pattern results in the same as the labeling pattern of methyl carbon of acetyl −CoA. Glutamate is converted into a-ketoglutarate and carbon a-ketoglutarate is present in the same position of methyl carbon of acetyl −CoA in the stage of the TCA cycle. In the first cycle, central carbons get labeled. In the second cycle, all the carbons will get a quarter of the label. In the first two cycles, the loss of radioactivity will not be observed. However, in later cycles, 50 % of loss of radioactivity is noticed in the level of each cycle.

Answer 13

Ch. 19 - Radiolabeling with 14C-Glutamate Describe the...Ch. 19 - Prob. 2P Ch. 19 - Assessing the Effect of Active-Site...Ch. 19 - Understanding the Mechanism of the -Ketoglutarate...Ch. 19 - Understanding the Action of Fluoroacetate on the...Ch. 19 - Prob. 6P Ch. 19 - Prob. 7P Ch. 19 - Prob. 8P Ch. 19 - Prob. 9P Ch. 19 - Prob. 10P

Radiolabeling with 14 C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at C y with 14 C.

Concept explainers

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 19 Solutions