Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 19.2, Problem 1COMQ
Summary Introduction
Introduction:
Replica plating is a technique which, is used to select mutants from a given population of wild type of colonies. It involves plating a culture on a master plate and then, transferring it to a plate containing a selection agent using a velvet sheet. This technique was developed by Esther and Joshua Lederberg.
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In the phage titer experiment, why did you plate multiple dilutions?
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Chapter 19 Solutions
Genetics: Analysis and Principles
Ch. 19.1 - 1. A mutation changes a codon that specifies...Ch. 19.1 - A down promoter mutation causes the promoter of a...Ch. 19.1 - 3. A mutation in one gene that reverses the...Ch. 19.1 - Which of the following is an example of a somatic...Ch. 19.2 - Prob. 1COMQCh. 19.3 - Which of the following is not an example of a...Ch. 19.3 - A point mutation could be caused by a....Ch. 19.3 - One way that TNRE may occur involves the formation...Ch. 19.4 - Nitrous acid replaces amino groups with keto...Ch. 19.4 - Prob. 2COMQ
Ch. 19.4 - Prob. 3COMQCh. 19.5 - The function of photolyase is to repair a....Ch. 19.5 - Which of the following DNA repair systems may...Ch. 19.5 - 3. In nucleotide excision repair in E. coli, the...Ch. 19.5 - Prob. 4COMQCh. 19.5 - An advantage of translesion-replicating...Ch. 19 - Is each of the following mutations a transition,...Ch. 19 - Prob. 2CONQCh. 19 - What does a suppressor mutation suppress? What is...Ch. 19 - Prob. 4CONQCh. 19 - X-rays strike a chromosome in a living cell and...Ch. 19 - Prob. 6CONQCh. 19 - Prob. 7CONQCh. 19 - 8. A point mutation occurs in the middle of the...Ch. 19 - Prob. 9CONQCh. 19 - Prob. 10CONQCh. 19 - 11. Is a random mutation more likely to be...Ch. 19 - 12. Which of the following mutations could be...Ch. 19 - Prob. 13CONQCh. 19 - Discuss the consequences of a germ-line versus a...Ch. 19 - Prob. 15CONQCh. 19 - Explain how a mutagen can interfere with DNA...Ch. 19 - What type of mutation (transition, transversion,...Ch. 19 - Explain what happens to the sequence of DNA during...Ch. 19 - Distinguish between spontaneous and induced...Ch. 19 - Prob. 20CONQCh. 19 - Prob. 21CONQCh. 19 - Prob. 22CONQCh. 19 - Trinucleotide repeat expansions (TNREs) are...Ch. 19 - 24. With regard to TNRE, what is meant by the term...Ch. 19 - 25. What is the difference between the mutation...Ch. 19 - Achondroplasia is a rare form of dwarfism. It is...Ch. 19 - Prob. 27CONQCh. 19 - In the treatment of cancer, the basis for many...Ch. 19 - Prob. 29CONQCh. 19 - 30. Which of the following examples is likely to...Ch. 19 - Prob. 31CONQCh. 19 - Prob. 32CONQCh. 19 - Prob. 33CONQCh. 19 - With regard to the repair of double-strand breaks,...Ch. 19 - Prob. 35CONQCh. 19 - Prob. 36CONQCh. 19 - 37. Three common ways to repair changes in DNA...Ch. 19 - Prob. 38CONQCh. 19 - Prob. 39CONQCh. 19 - Explain how the technique of replica plating...Ch. 19 - 2. Outline how you would use the technique of...Ch. 19 - 3. From an experimental point of view, is it...Ch. 19 - Prob. 4EQCh. 19 - Prob. 5EQCh. 19 - 6. Richard Boyce and Paul Howard-Flanders...Ch. 19 - In E. coli, a variety of mutator strains have been...Ch. 19 - 2. Discuss the times in a person’s life when it is...Ch. 19 - A large amount of research is aimed at studying...
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- In the experiment of Avery, McLeod, and McCarty, the additionof RNase or protease to a DNA extracta. prevented the conversion of type S bacteria into type R bacteria.b. allowed the conversion of type S bacteria into type R bacteria.c. prevented the conversion of type R bacteria into type S bacteria.d. allowed the conversion of type R bacteria into type S bacteria.arrow_forwardIn another experiment, Griffith mixed heat killed S strain with live R strain bacteria and injected the mixture into mice. A. What strain of the bacteria was found in the blood samples of the mice? B. What were the results of this experiment? C. What conclusion did he reach based on these results?arrow_forwardUsing the data in Table, identify restriction enzymes that (a) produce blunt ends; (b) recognize and cleave the same sequence (called isoschizomers); (c) produce identical sticky ends.arrow_forward
- All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1 phage except: A) it served as an example of an inflexible test B) it involved looking for T1-resistant bacteria as an end measure C) it showed that numbers of mutant organisms observed after T 1 phage were added to the culture tended to vary from experiment to experiment D) it rejected the possibility of adaptive mutations E) it supported the concept of spontaneous mutation ratesarrow_forwardGriffith discovered a “transforming factor” in bacteria that conveyed infectiousness in 1928. In 1944, Avery, MacLeod and McCarty showed that the transforming factor is DNA A. Both statements are correct B. Both statements are incorrect C. First statements is correct. Second statement is incorrect D. First statements is incorrect. Second statement is correctarrow_forwardSome scientists are trying to engineer bacteriophageto treat bacterial infections in humans when theinfections do not respond to chemical antibiotics.a. What possible advantages might phage therapyhave over antibiotic therapy?b. Describe potential difficulties that would need tobe overcome for phage therapy to succeed.c. How might researchers best confront the issue thatbacterial cells could become resistant to bacteriophage just as they could to antibiotics?arrow_forward
- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhen you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful? a. the dilution that produces the plate with no visible virus b. the plate with fewer than 25 viral infections of E. coli c. the plate with the most dense virus d. the plate with between 30 and 300 visible viral infections of E. coliarrow_forwardThe activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forward
- Following are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.arrow_forwardA transducing particle injects all of the DNA that it contains into a bacterial cell. Which of the following are TRUE? A. The bacterium becomes Hfr B. The bacterium becomes F+ C. The bacterium immediately acquires new functions as the injected DNA is rapidly transcribed and translated into mRNA and proteins D. This injected DNA can integrate into the bacterium’s genome through a process called recombinationarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
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