Campbell Essential Biology (7th Edition)
Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 2, Problem 12IMT

For each statement, identify which major theme is evident (the relationship of structure to function, information flow, pathways that transform energy and matter, interactions within biological systems, or evolution) and explain how the statement relates to the theme. If necessary, review the theme descriptions (see Chapter 1) and review the examples highlighted in blue in this chapter.

12. Cells are constantly rearranging molecules by breaking existing chemical bonds and forming new ones.

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a) What 5 parts must be present in a plasmid that is to be used for vector cloning?   b) What is a multiple cloning site and why is it important that this site fall within a selectable marker gene?   c) In your own words, define “vector” in the context of vector cloning.
a) What is the difference between blunt ends and sticky ends and which one is useful for vector cloning?   b) There are also possible outcomes in terms of the attempt to get the bacteria to pick up the recombinant plasmid: Some bacteria will not pick up the plasmid Some bacteria will pick up the recombinant plasmid. Describe how scientists target ONLY the bacteria that have the recombinant plasmid.   c) IPTE xgal is a lactose analog that Is used in lactose free milk Is used to prevent growth of bacteria that do not have the beta galactosidase gene Is used to differentiate bacteria that have picked up a target DNA within their plasmids from those that have not Helps with bacterial transformation
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