BuyFind

Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135
BuyFind

Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135

Solutions

Chapter
Section
Chapter 2, Problem 40RE
Textbook Problem

MATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and with pH 8.0: dissolve 2.02 g of TRIS (free base, MW = 121.1 g/mol) and 5.25 g of TRIS hydrochloride (the acidic form, MW = 157.6 g/mol) in a total volume of 1 L. Verify that this recipe is correct.

Expert Solution
Interpretation Introduction

Interpretation:

The given recipe is to be verified.

Concept information:

Buffers are the solutions that have the capability of resisting any kind of change.

On the other hand, buffer systems are the solutions that are capable of resisting any change in the pH if added with some amount of a strong base or a strong acid.

The Henderson–Hasselbalch equation states the relationship between the pKa value of an acid and the pH of a solution that contains an acid along with its conjugate base. The equation is as follows:

pH=pKa+log[A-HA] 

Explanation of Solution

Given information: A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and with pH 8.0: dissolve 2.02 g of TRIS(free base, MW=121.1 g/mol) and 5.25 g of TRIS hydrochloride (the acidic form, MW=157.6 g/mol) in a total volume of 1 L.

Calculating the molar amount of the two forms as follows:

The molecular weight (MW) of TRIS =121.1 g/mol

Therefore, the number of moles of TRIS is calculated as follows:

Number of moles=2.02 g121.1 g/mol=0.0167 mole

The molecular weight (MW) of TRIS hydrochloride =157.6 g/mol

Therefore, the number of moles of TRIS hydrochloride is calculated as follows:

Number of moles=5.25 g157.6 g/mol=0

Want to see this answer and more?

Bartleby provides explanations to thousands of textbook problems written by our experts, many with advanced degrees!

See solution

Chapter 2 Solutions

Biochemistry
Show all chapter solutions
Ch. 2 - RECALL What are some macromolecules that have...Ch. 2 - BIOCHEMICAL CONNECTIONS How are hydrogen bonds...Ch. 2 - REFLECT AND APPLY Rationalize the fact that...Ch. 2 - REFLECT AND APPLY Draw three examples of types of...Ch. 2 - RECALL What are the requirements for molecules to...Ch. 2 - REFLECT AND APPLY Many properties of acetic acid...Ch. 2 - REFLECT AND APPLY How many water molecules could...Ch. 2 - REFLECT AND APPLY Both RNA and DNA have negatively...Ch. 2 - RECALL Identify the conjugate acids and bases in...Ch. 2 - RECALL Identify conjugate acids and bases in the...Ch. 2 - REFLECT AND APPLY Aspirin is an acid with a pKa of...Ch. 2 - RECALL Why does the pH change by one unit if the...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydroxide ion...Ch. 2 - RECALL Define the following: (a) Acid dissociation...Ch. 2 - REFLECT AND APPLY Look at Figure 2.17. If you did...Ch. 2 - BIOCHEMICAL CONNECTIONS List the criteria used to...Ch. 2 - BIOCHEMICAL CONNECTIONS What is the relationship...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the ratio of TRIS/TRIS-H+ in...Ch. 2 - MATHEMATICAL What is the ratio of HEPES/HEPES-H+...Ch. 2 - MATHEMATICAL How would you prepare 1 L of a 0.050...Ch. 2 - MATHEMATICAL The buffer needed for Question 35 can...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL A catalog in the lab has a recipe for...Ch. 2 - MATHEMATICAL If you mix equal volumes of 0.1 M HCl...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL If you have 100 mL of a 0.10 M TRIS...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL Show that, for a pure weak acid in...Ch. 2 - MATHEMATICAL What is the ratio of concentrations...Ch. 2 - BIOCHEMICAL CONNECTIONS You need to carry out an...Ch. 2 - MATHEMATICAL If the buffer suggested in Question...Ch. 2 - BIOCHEMICAL CONNECTIONS Suggest a suitable buffer...Ch. 2 - BIOCHEMICAL CONNECTIONS Which of the buffers shown...Ch. 2 - MATHEMATICAL The solution in Question 35 is called...Ch. 2 - REFLECT AND APPLY In Section 2-4, we said that at...Ch. 2 - MATHEMATICAL Define buffering capacity. How do the...Ch. 2 - BIOCHEMICAL CONNECTIONS If you wanted to make a...Ch. 2 - BIOCHEMICAL CONNECTIONS We usually say that a...Ch. 2 - RECALL What quality of zwitterions makes them...Ch. 2 - REFLECT AND APPLY Many of the buffers used these...Ch. 2 - REFLECT AND APPLY Another characteristic of modern...Ch. 2 - REFLECT AND APPLY Identify the zwitterions in the...Ch. 2 - BIOCHEMICAL CONNECTIONS A frequently recommended...

Additional Science Textbook Solutions

Find more solutions based on key concepts
Show solutions
Water-soluble vitamins are mostly absorbed into the lymph. the blood. the extracellular fluid, b and c.

Nutrition: Concepts and Controversies - Standalone book (MindTap Course List)

An inductor in the form of a solenoid contains 420 and is 16.0 cm in length. A uniform rate of decrease of curr...

Physics for Scientists and Engineers, Technology Update (No access codes included)

What advantages do scientific names have over common names?

Oceanography: An Invitation To Marine Science, Loose-leaf Versin