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BiochemistryBiochemistryMATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and with pH 8.0: dissolve 2.02 g of TRIS (free base, MW = 121.1 g/mol) and 5.25 g of TRIS hydrochloride (the acidic form, MW = 157.6 g/mol) in a total volume of 1 L. Verify that this recipe is correct.BuyFind*arrow_forward*

9th Edition

Mary K. Campbell + 2 others

Publisher: Cengage Learning

ISBN: 9781305961135

Chapter 2, Problem 40RE

Textbook Problem

MATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 *M* and with pH 8.0: dissolve 2.02 g of TRIS (free base,

Expert Solution

Interpretation Introduction

**Interpretation:**

The given recipe is to be verified.

**Concept information:**

Buffers are the solutions that have the capability of resisting any kind of change.

On the other hand, buffer systems are the solutions that are capable of resisting any change in the pH if added with some amount of a strong base or a strong acid.

The Henderson–Hasselbalch equation states the relationship between the

**Given information:** A catalog in the lab has a recipe for preparing

Calculating the molar amount of the two forms as follows:

The molecular weight (MW) of TRIS

Therefore, the number of moles of TRIS is calculated as follows:

The molecular weight (MW) of TRIS hydrochloride

Therefore, the number of moles of TRIS hydrochloride is calculated as follows:

Biochemistry

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