Concept explainers
MATHEMATICAL Define buffering capacity. How do the following buffers differ in buffering capacity? How do they differ in pH?
Buffer a: 0.01 M
Buffer b: 0.10 M
Buffer c: 1.0 M
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Biochemistry
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- REFLECT AND APPLY Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.arrow_forwardMATHEMATICAL Consider the reaction AB+C, where G=0.00. (a) What is the value of G (not G) when the initial concentrations of A, B, and C are 1 M, 103M,and106M? (b) Try the same calculations for the reaction D+EF, for the same relative order of concentrations. (c) Try the same calculations for the reaction GH, if the concentrations are 1Mand103M for G and H, respectively.arrow_forwardMATHEMATICAL Calculate the pH of a buffer solution prepared by mixing 75 mL of 1.0 M lactic acid (see Table 2.6) and 25 mL of 1.0 M sodium lactate.arrow_forward
- REFLECT AND APPLY It is frequently said that camels store water in their humps for long desert journeys. How would you modify this statement on the basis of information in this chapter?arrow_forwardREFLECT AND APPLY Why is it advantageous for a cell to have organelles? Discuss this concept from the standpoint of thermodynamics.arrow_forwardMATHEMATICAL If you mix equal volumes of 0.1 M HCl and 0.20 M TRIS (free amine form; see Table 2.8), is the resulting solution a buffer? Why or why not?arrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning