Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 6P

The weak acid HA is 2% ionized (dissociated) in a 0.20 M solution.
a. What is Ka for this acid?
b. What is the pH of this solution?

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  • A 0.10 M solution of the deadly poison hydrogen cyanide, HCN, has a pH of 5.2. Calculate the [H3O+] of the solution. Is HCN a strong or a weak acid?
    Consider a buffer solution that contains 0.55 M NH2CH2CO2H and 0.35 M NH2CH2CO2Na. pKa(NH2CH2CO2H)=9.88. a. Calculate its pH. b. Calculate the change in pH if 0.155 g of solid NaOH is added to 250 mL of this solution. c. If the acceptable buffer range of the solution is ±0.10 pH units, calculate how many moles of H3O+ can be neutralized by 250 mL of the initial buffer.
    The ionization of p-nitrophenol is shown below (pKa = 7.0): a. Identify the weak acid and conjugate base. b. At pH 7, what are the relative concentrations of ionized and un-ionized p-nitrophenol? c. If enough concentrated hydrochloric acid is added to a solution of p-nitrophenol to lower the pH from 7 to 5, what will happen to the relative concentrations of the ionized and un-ionized forms? d. Ionized p-nitrophenol has a yellow color, while the un-ionized form is colorless.  The yellow color can be measured using a spectrophotometer at 400nm.  In order to determine the total amount of p-nitrophenol in a solution, would you perform the spectrophotometer reading at an acidic or basic pH?  Clearly explain why? e. A solution of p-nitrophenol at pH 7.95 was found to have an A400 of 0.255 .  What is the total concentration (in µM) of p-nitrophenol (ionized plus un-ionized) in the solution?  The molar extinction coefficient of p-nitrophenol is 18,500 M-1cm-1 and the pKa is 7.
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