Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN: 9781305960060
Author: Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher: Cengage Learning
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Chapter 20, Problem 20.32E
When handling or storing solutions of enzymes, the
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Describe three effects of pH on ionization of a weak acid or base that impact biological stability of a protein.
An enzyme is needed to be stored in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory:
Buffer System
Weak Acid Component
Ka
Conjugate Base Salt
PAH
Benzoic acid, C6H5COOH
6.25 x 10-5
NaC6H5COO
SAD
Boric acid, H3BO3
5.81 x 10-7
NaH2BO3
DOH
Hydrogen cyanide, HCN
6.20 X 10-10
NaCN
In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? (show calculations and briefly explain)
Write the equilibrium reaction involved in the buffer system.
Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
What does high NaCl do to protein that is bound to a Sp column?
Chapter 20 Solutions
Chemistry for Today: General, Organic, and Biochemistry
Ch. 20 - What is the role of enzymes in the body?Ch. 20 - List two ways that enzyme catalysis of a reaction...Ch. 20 - What is the relationship between an enzyme and the...Ch. 20 - Why are so many different enzymes needed?Ch. 20 - Define what is meant by the term enzyme...Ch. 20 - Prob. 20.6ECh. 20 - Prob. 20.7ECh. 20 - Prob. 20.8ECh. 20 - What is the relationship between urea and urease?...Ch. 20 - Match the following enzymes and substrates: Enzyme...
Ch. 20 - Match the following general enzyme names and...Ch. 20 - Prob. 20.12ECh. 20 - Some enzymes consist of protein plus another...Ch. 20 - Prob. 20.14ECh. 20 - Prob. 20.15ECh. 20 - Prob. 20.16ECh. 20 - Prob. 20.17ECh. 20 - Prob. 20.18ECh. 20 - Prob. 20.19ECh. 20 - How is enzyme specificity explained by the...Ch. 20 - Prob. 20.21ECh. 20 - Prob. 20.22ECh. 20 - Prob. 20.23ECh. 20 - What observations may be used in experiments to...Ch. 20 - Prob. 20.25ECh. 20 - Prob. 20.26ECh. 20 - Prob. 20.27ECh. 20 - Write a single sentence to summarize the...Ch. 20 - Prob. 20.29ECh. 20 - Prob. 20.30ECh. 20 - Prob. 20.31ECh. 20 - When handling or storing solutions of enzymes, the...Ch. 20 - Prob. 20.33ECh. 20 - Prob. 20.34ECh. 20 - Prob. 20.35ECh. 20 - List an antidote for each of the two poisons in...Ch. 20 - Prob. 20.37ECh. 20 - Prob. 20.38ECh. 20 - Describe the importance of zymogens in the body....Ch. 20 - Prob. 20.40ECh. 20 - Prob. 20.41ECh. 20 - Name and contrast the two types of modulators.Ch. 20 - Prob. 20.43ECh. 20 - Prob. 20.44ECh. 20 - Prob. 20.45ECh. 20 - Prob. 20.46ECh. 20 - Prob. 20.47ECh. 20 - Prob. 20.48ECh. 20 - Prob. 20.49ECh. 20 - Prob. 20.50ECh. 20 - Prob. 20.51ECh. 20 - Prob. 20.52ECh. 20 - Prob. 20.53ECh. 20 - Prob. 20.54ECh. 20 - Explain how the pasteurization of milk utilizes...Ch. 20 - Prob. 20.56ECh. 20 - Prob. 20.57ECh. 20 - Prob. 20.58ECh. 20 - Prob. 20.59ECh. 20 - Why are enzymes that are used for laboratory or...Ch. 20 - Describe the difference between graphs showing...Ch. 20 - Answer the question associated with Figure 20.3....Ch. 20 - Prob. 20.63ECh. 20 - Prob. 20.64ECh. 20 - Prob. 20.65ECh. 20 - Prob. 20.66ECh. 20 - Prob. 20.67ECh. 20 - Which of the following is not a characteristic of...Ch. 20 - The human body has an average pH of about 7 and a...Ch. 20 - Look at Table 20.4 and identify the proteolytic...Ch. 20 - Saliva contains mucus, water, and _______, which...Ch. 20 - Most human enzymes function best in the...Ch. 20 - The site on an enzyme molecule that does the...Ch. 20 - The process by which an enzyme acts on the...Ch. 20 - In the multienzyme sequence shown below, molecules...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Similar questions
Write the chemical equilibrium and expression for the equilibrium constants for the ionization of (a) tartaric acid. (b) malic acid.
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How is enzyme specificity explained by the lock-and-key theory?
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Describe the difference between graphs showing temperature versus reaction rate for an enzyme-catalyzed reaction and an uncatalyzed reaction.
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Explain why a protein is least soluble in an aqueous medium that has a pH equal to the isoelectric point of the protein.
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Apatite, Ca5(PO4)3OH, is the mineral in teeth.
On a chemical basis explain why drinking milk strengthens young children’s teeth.
Sour milk contains lactic acid. Not removing sour milk from the teeth of young children can lead to tooth decay. Use chemical principles to explain why.
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"All enzymes are proteins, not all proteins are enzymes" Explain this statement ?
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When the terminal phosphate is cleaved from the ADP molecule, the products are adenosine diphosphate and inorganic phosphate:
ATP + H2O ––> ADP + Pi
where Pi stands for the various forms of phosphate that exist at the pH of the particular solution. If the total concentration of Pi is 2.5 x 10–2 M, what are the concentrations of the predominant forms of phosphate that exist at pH 7.4?
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I don’t understand how to do part b of question 5. The other picture is my work of part a. How would changing the enzyme concentration affect Km and Vmax. The answer given to us is Vo= 0.796mM/s. I just don’t understand how to get to that answer.
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a. Explain why the presence of certain proteins in body fluids such as urine or blood can indicate that cellular damage has occurred in the body.
b. Explain why a protein is least soluble in an aqueous medium that has a pH equal to the isoelectric point of the protein.
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Given that the solubility reaction for calcium phosphate is
Ca3(PO4)2(s)⇌3Ca2+(aq)+2PO43−(aq)Ca3(PO4)(s)⇌3Ca2+(aq)+2PO43−(aq)
why does the addition of acid increase the solubility of calcium phosphate?
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Given that C6H11COOH has a pKa = 4.8 and C6H11N + H3 has a pKa = 10.7, what pH would you make the water layer to cause both compounds to dissolve in it?
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What is the actual chemical component for an anthocyanin (assume a cyanidin structure), and a carotenoid (assume a lutein structure), and indicate what is the common feature of these indicators that could act as an acid or base. (While the structures are complicated molecules, compare with acetic acid, CH3COOH, a weak acid).
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY