Chemistry for Today: General, Organic, and Biochemistry
Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN: 9781305960060
Author: Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher: Cengage Learning
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Textbook Question
Chapter 20, Problem 20.32E

When handling or storing solutions of enzymes, the pH is usually kept near 7.0 . Explain why.

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Describe three effects of pH on ionization of a weak acid or base that impact biological stability of a protein.
An enzyme is needed to be stored in a buffer solution with a pH of 10.00. Unfortunately, only three buffer systems are available in their laboratory: Buffer System Weak Acid Component Ka Conjugate Base Salt PAH Benzoic acid, C6H5COOH 6.25 x 10-5 NaC6H5COO SAD Boric acid, H3BO3 5.81 x 10-7 NaH2BO3 DOH Hydrogen cyanide, HCN 6.20 X 10-10 NaCN   In what buffer system (PAH, SAD, or DOH) will Phoebe store the enzyme? (show calculations and briefly explain) Write the equilibrium reaction involved in the buffer system. Calculate the amount (in mL) of the 2.00 M stock solution (pH 10.00) of the same buffer system needed to prepare 250 mL of a 0.650 M buffer pH 10.00.
What does high NaCl do to protein that is bound to a Sp column?

Chapter 20 Solutions

Chemistry for Today: General, Organic, and Biochemistry

Ch. 20 - Match the following general enzyme names and...Ch. 20 - Prob. 20.12ECh. 20 - Some enzymes consist of protein plus another...Ch. 20 - Prob. 20.14ECh. 20 - Prob. 20.15ECh. 20 - Prob. 20.16ECh. 20 - Prob. 20.17ECh. 20 - Prob. 20.18ECh. 20 - Prob. 20.19ECh. 20 - How is enzyme specificity explained by the...Ch. 20 - Prob. 20.21ECh. 20 - Prob. 20.22ECh. 20 - Prob. 20.23ECh. 20 - What observations may be used in experiments to...Ch. 20 - Prob. 20.25ECh. 20 - Prob. 20.26ECh. 20 - Prob. 20.27ECh. 20 - Write a single sentence to summarize the...Ch. 20 - Prob. 20.29ECh. 20 - Prob. 20.30ECh. 20 - Prob. 20.31ECh. 20 - When handling or storing solutions of enzymes, the...Ch. 20 - Prob. 20.33ECh. 20 - Prob. 20.34ECh. 20 - Prob. 20.35ECh. 20 - List an antidote for each of the two poisons in...Ch. 20 - Prob. 20.37ECh. 20 - Prob. 20.38ECh. 20 - Describe the importance of zymogens in the body....Ch. 20 - Prob. 20.40ECh. 20 - Prob. 20.41ECh. 20 - Name and contrast the two types of modulators.Ch. 20 - Prob. 20.43ECh. 20 - Prob. 20.44ECh. 20 - Prob. 20.45ECh. 20 - Prob. 20.46ECh. 20 - Prob. 20.47ECh. 20 - Prob. 20.48ECh. 20 - Prob. 20.49ECh. 20 - Prob. 20.50ECh. 20 - Prob. 20.51ECh. 20 - Prob. 20.52ECh. 20 - Prob. 20.53ECh. 20 - Prob. 20.54ECh. 20 - Explain how the pasteurization of milk utilizes...Ch. 20 - Prob. 20.56ECh. 20 - Prob. 20.57ECh. 20 - Prob. 20.58ECh. 20 - Prob. 20.59ECh. 20 - Why are enzymes that are used for laboratory or...Ch. 20 - Describe the difference between graphs showing...Ch. 20 - Answer the question associated with Figure 20.3....Ch. 20 - Prob. 20.63ECh. 20 - Prob. 20.64ECh. 20 - Prob. 20.65ECh. 20 - Prob. 20.66ECh. 20 - Prob. 20.67ECh. 20 - Which of the following is not a characteristic of...Ch. 20 - The human body has an average pH of about 7 and a...Ch. 20 - Look at Table 20.4 and identify the proteolytic...Ch. 20 - Saliva contains mucus, water, and _______, which...Ch. 20 - Most human enzymes function best in the...Ch. 20 - The site on an enzyme molecule that does the...Ch. 20 - The process by which an enzyme acts on the...Ch. 20 - In the multienzyme sequence shown below, molecules...
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