Concept explainers
Interpretation:
The number of copies of target DNA after 12 cycles of polymerase chain reaction has to be calculated.
Concept Introduction:
Polymerase chain reaction (PCR) is widely used to amplify DNA sequences to produce millions of copies of a specific DNA segment. PCR technique finds applications for specific and rapid diagnosis of infectious diseases.
The various steps involved in PCR technique is as follows:
Stage 1: In the first stage, high temperature
Stage 2: In the next step, annealing is done at a temperature of about
Stage 3: In the third stage, two DNA strands obtained after annealing serve as a template for the DNA polymerase, and the enzyme then begins to extend the primers in both directions at about
This cycle is repeated for about 35 times until the target DNA region gets copied.
Each successive cycle of a polymerases chain reaction double the gene as illustrated below:
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