Human Biology (MindTap Course List)
11th Edition
ISBN: 9781305112100
Author: Cecie Starr, Beverly McMillan
Publisher: Cengage Learning
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Chapter 21, Problem 10SQ
Summary Introduction
Introduction: Recombinant DNA is synthesized as a part of
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Restriction enzymes found in bacterial cells are ordinarily useda. during DNA replication.b. to degrade the bacterial cell’s DNA.c. to degrade viral DNA that enters the cell.d. to attach pieces of DNA together.
Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide.
B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube.
D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
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Original DNA template: 3'-ACGGTCAATTTGCTG-5
a) Transcribe the sequence. b) Translate the sequence. c) What type of mutation is present in the strand 3 '- ACGGTCAATATTGCTG - 5
d) Provide the entire mutated sequence of amino acids.
e) Explain the effect that this mutation will have.
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- n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesarrow_forwardMatch each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___ Ti plasmid b. alleles commonly contain them ___ cDNA synthesis c. a persons unique collection of short tandem repeats ___ SNP d. requires reverse transcriptase ___ transgenic e. cuts DNA ___ restriction enzyme f. used in plant gene transfersarrow_forwardAfter a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.arrow_forward
- Why must the lagging strand of DNA be replicated in short pieces a. Because of limited space b. To make proofreading of code easier . C. Otherwise, the helix will become distorted . D. The DNA polymerase can synthesize in only one directionarrow_forwardDNA strands are anti-parallel and DNA polymerase can only synthesize DNA in a 5' to 3' direction. How does the enzyme synthesize both strands at the same time? A. The leading strand is sythesised in Okazaki fragments B. The lagging strand is synthesised in short Okazaki fragments. C. Only one strand is replicated. D. There are more than one DNA polymerase involved.arrow_forwardWhich of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.arrow_forward
- Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organismarrow_forwardYou used agarose gel electrophoresis to separate DNA fragments of different size and the experiment worked well. However, you wanted to re run the experiment but this time you made the gel with a higher percentage of Agarose. How might this affect your results compared to the first run? a. There would be no difference between the runs since it is the current, not the agarose that causes migration. b. The higher concentration of agarose would cause the DNA to break apart. c. You can't predict how the concetration of agarose would affect migration. d. The DNA fragments would migrate further down the gel than they did the first time. e. The DNA fragments wouldn't migrate as far down the gel as they did the first time.arrow_forwardBase analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.arrow_forward
- DNA cloning isa. making multiple genetically identical cells.b. making multiple copies of a piece of DNA.c. inserting DNA into a cell.d. changing the nucleotide sequence of a strand of DNA.arrow_forwarda. Explain why the PCR is unlikely to amplify contaminatingbacterial DNA in a sample of human DNA. b. Explain how PCR could be used to pick a gene out of a complexgenome and amplify it.arrow_forward1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequencesarrow_forward
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