Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 21.1, Problem 1TQ
Summary Introduction
To review:
The selection of optimal and suitable DNA (Deoxyribonucleic acid) extraction method for performing a metagenomic analysis of soil samples taken from various parts of wetlands.
Introduction:
Metagenomics involves the study of genetic material that is recovered directly from the environment. Analysis of metagenomic sequencing has posed severe challenges to most of the scientific researchers as this analysis has to follow a complex structure of steps. While doing so the researchers have to face environmental oriented hazards and also have to choose an appropriate target community.
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There are many other applications in which DNA barcoding might be useful. For example, how might ecologists use DNA barcoding to find out what species are present in a rain forest, or what kinds of animals a predator eats?
If the PCR analysis of your sample did not work can you suggest why? How can you test if this is true?
What would you conclude if you attained nested PCR product for the genomic Arabidopsis control, but not your plant sample? Give one reasonable explanation and be specific.
Chapter 21 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 21.1 - Prob. 1TQCh. 21.1 - Prob. 2TQCh. 21.1 - Prob. 3TQCh. 21.2 - Prob. 1TQCh. 21.4 - Prob. 1TQCh. 21.4 - Prob. 2TQCh. 21.4 - Prob. 3TQCh. 21.5 - Prob. 1TQCh. 21.6 - Prob. 1TQCh. 21.6 - Prob. 2TQ
Ch. 21.6 - Prob. 3TQCh. 21.6 - Prob. 4TQCh. 21 - Prob. 1RQCh. 21 - Prob. 2RQCh. 21 - Prob. 3RQCh. 21 - Prob. 4RQCh. 21 - Prob. 5RQCh. 21 - Prob. 6RQCh. 21 - Prob. 7RQCh. 21 - Prob. 8RQCh. 21 - Prob. 9RQCh. 21 - Prob. 10RQCh. 21 - Prob. 11RQCh. 21 - Prob. 12RQCh. 21 - Prob. 13RQCh. 21 - Prob. 14RQCh. 21 - Prob. 1TQCh. 21 - Prob. 2TQCh. 21 - Prob. 3TQ
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- make a scatter plot with the given criteria and data.arrow_forwardAssuming the 35S promotor sequence is 500 base pairs long, with the electrophoresis results, what would you expect to find from a GMO food? A non-GMO food?arrow_forwardCould the PCR sample loaded on lane 4 be considered a successful PCR?arrow_forward
- What is metaproteomics? How does it differ from metagenomics? In what ways are the two experimental approaches used in microbial ecology?arrow_forwardIf a DNA sample was GC-rich, what would you need to do to adjust your PCR reaction and why?arrow_forwardIn your opinion, is antibiotic use at farm sites promote the development and spread of antibiotic resistant genes in bacteria? Why or why not please explain based on the articlearrow_forward
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- In what way could the extreme sensitivity of the PCR method be aproblem when working with clinical specimens?arrow_forwardCan you suggest a method to remove oil(hydrocarbon)from seeds based on your understanding of rDNA technology and chemistry of oil?arrow_forwardsuggest a strategy that you might employ to isolate all of the genes involved in nitrogen fixation from Azobacter vinelandi assuming that you do not have nif genes from other microorganisms to use as hybridization probes and discuss whether it is possible to genetically engineer plants to fix nitrogen.arrow_forward
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