Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.5, Problem 1CS
Summary Introduction
To propose: A model that can determine the outcome of transposition depending on the pattern of replication.
Introduction: DNA is a type of
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VISUAL SKILLS Compare Figure 20.7 with Figure 16.20.How does replication of DNA ends during PCR proceedwithout shortening the fragments each time?
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Activity 1.4: Essay.Direction: Explain your answer.Today, it is easy to make transgenic plants and animals. What are some important safety and ethical issues raised by this use of recombinant DNA technology? Explain your answer.
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- Compare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the same gene cloned by reverse transcriptase PCR (RTPCR).arrow_forwardQ. Based on your understanding of plasmids’ answer the following questions.1. Why is a daughter cell that fails to inherit a kind of plasmid dies? 2. How transfer of Col E1 plasmid to an F cell occurs? 3. What component you will must include in constructing a plasmid cloning vector that can replicate in both bacteria and yeast? 4. How copy number of a plasmid may affect its segregation? 5. How the yeast 2u circle maintains 80 copies in a cell?arrow_forwardPlease help witht this homework question A- What are the topological parameters (linking number, twist, and writhe)for a relaxed, 4,200 base pair circular double-stranded DNA plasmid? LK=? Tw= ? Wr=? The CRISPR-Cas9 protein first forms a protein-RNA complex with a guide RNA, and then binds to DNA sequences that match a 20-base target sequence within the guide. When the CRISPR-Cas9 complex binds to DNA, it unwinds about 20 base pairs of the DNA double helix. B- Why is DNA unwinding required for CRISPR-Cas9 protein-RNA complexto recognize target sites? C-How would the topological parameters (linking number, twist, andwrithe) of the 4,200 bp plasmid in (A) change when the CRISPR-Cas9 complex binds the DNA and unwinds about 20 bp? Lk=? Tw=? Wr=? D- What is the linking number after the CRISPR-Cas9 complex cleaves theDNA?arrow_forward
- Pls Help me. 2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type of medium will be used? How will it work?arrow_forward24. In recombinant DNA technology, genes are usually inserted into what form of DNA before being transferred into bacteria? - [ ] Teddy bear - [ ] Nucleus - [ ] Transposon - [ ] Ribosome - [ ] Plasmid 25. In PCR , what is happening at the denaturation step (94 )? - [ ] RNA polymerase creates a new RNA strand . - [ ] DNA strands come apart . - [ ] DNA polymerase creates a new DNA strand. - [ ] Primers bind to the target DNA. - [ ] Nucleotides bind to the primer .arrow_forwardTOPIC: PCR and Gene Cloning Basics Question: What are 2 possible roles of CaCl2 in the transformation process?arrow_forward
- Expand PCR? Describe the different Steps involved in this technique?arrow_forwardQ1: Summarize how DNA repair works and why the repair mechanisms are essential for the normal function of cells and whole organisms. Q2: Is DNA repair 100 percent effective? Q3: What would happen to an organism if its DNA repair became less effective?arrow_forwardQuestion:- When mapping sequence reads to a reference chromosome, discuss the parameters that determine whether a read maps to a specific chromosomal location ?arrow_forward
- Describe, in detail, causes for mutations that occur during replication. For each, use detail to describe how the mutation would occur, classify the type of mutation that results, and the effect it may have on the cell. What are two causes for mutations during replication? What is one cause for mutations at the end of replication? Differentiate between transition and transversion mutations. What are they, examples? How do point deletions/insertions lead to frameshift mutations- your answer should include what a “frame” is, what a codon is and how codons are responsible for making aa chains?arrow_forwardQuick help Only cell biology Which process is described in the following paragraph? During DNA synthesis, before the enzyme adds the next nucleotide to a growing DNA strand, it checks whether the previously added nucleotide is correctly base-paired to the template strand. If so, the polymerase adds the next nucleotide; if not, the polymerase clips off the mispaired nucleotide and tries again. This is carried out by cleaving the phosphodiester backbone using the enzyme’s 3’-to-5’ exonuclease activity.arrow_forwardPlease answer fast 1. which of the following statements is true of no -homologous end joining? a. it's error free b. it's a double strand repair pathway c. it requires RNA Polymerase d. it utilizes the sister chromatid as a template for repair 2. lab technique used to see how many different places a specfic probe would bind to the DNA in an organism genome. a. southern blot b. western blot c. situ hybridization d. reverse transcriptase qPCRarrow_forward
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