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Chemistry & Chemical Reactivity

10th Edition
John C. Kotz + 3 others
ISBN: 9781337399074

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BuyFindarrow_forward

Chemistry & Chemical Reactivity

10th Edition
John C. Kotz + 3 others
ISBN: 9781337399074
Textbook Problem

Care must be exercised in selecting good primers to use in PCR.

  1. a. Why are primers often chosen that have a high number of cytosines and guanines in their sequences?
  2. b. Why would a primer sequence of CCCCCCCCCCGGGGGGGGGG not be a good choice?
  3. c. Why is a vast excess of primer used for the amount of DNA to be amplified?

(a)

Interpretation Introduction

Interpretation:

The primers frequently chosen that have high number of cytosines and guanines in their sequences; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

Explanation

Primers often choosen that have high number of Cytosine and Guanine because Guanine and Cytosine bind with three hydrogen bonds and Adenine and Thymine bind with two hydrogen bonds per base pair respectively...

(b)

Interpretation Introduction

Interpretation:

The primer sequence of CCCCCCCCCCGGGGGGGGGG not is a good choice; the reason has to be predicted.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

(c)

Interpretation Introduction

Interpretation:

Vast excess of primer used for the amount of DNA has to be amplified.

Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.

Primers are short strand(18-20 base pairs) of DNA and RNA which helps in initition of DNA synthesis. The main role of primer is to allow polymerase to start because it cannot start by itself.

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