Introduction to General, Organic and Biochemistry
11th Edition
ISBN: 9781285869759
Author: Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher: Cengage Learning
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Question
Chapter 25, Problem 25.116P
Interpretation Introduction
Interpretation:
Interpret the way to amplify DNA which require dna polymerase stable in heat.
Concept Introduction:
Polymerisation of Dna requires the heat stable enzyme to withstand at that high temperature. Along with it, the Dna primer goes through 30 repeating cycle of polymerisation to generate multiple copies of DNA. The number of Dna generated reach to millions of time. The strand moves from 5’ to 3’ direction opposite to the base sequence of given strand.
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Check out a sample textbook solutionStudents have asked these similar questions
temperature of 45 °C for 15 seconds in a PCR reaction is needed for
A The two strands of DNA open
B. The primers bind to the ends of the gene to be amplified
C. polymerase can bind
D. The strand is extended at temperatures that do not damage DNA
Explain your answer with explanation
For the polymerase chain reaction to occur, ____.
a.
a large sample of DNA is needed
b.
a sticky end must be available for the ligase enzyme to function
c.
the DNA must remain double stranded
d.
the reaction temperature must be cycled from low to high
e.
all DNA fragments must be identical
Replication of DNA produces two daughter DNA molecules in which
a. once daughter molecules contains both parent strands and one daughter molecule contains both newly synthesized strands
b. each daughter molecule contians one parent strand and one newly synthesized strand
c. each daughter molecule contians two newly synthesized strands
d. each daughter molecule contians both parent strands
Chapter 25 Solutions
Introduction to General, Organic and Biochemistry
Ch. 25.2 - Problem 25-1 Draw the structure of UMP.Ch. 25 - 25-2 What structures Of the cell, visible in a...Ch. 25 - 25-3 Name one hereditary disease.Ch. 25 - 25-4 What is the basis unit of heredity?Ch. 25 - 25-5 (a) Where in a cell is the DNA located? (b)...Ch. 25 - 25-6 What are the components of (a) a nucleotide...Ch. 25 - 25-7 What are the differences between DNA and RNA?Ch. 25 - 25-8 Draw the structures of ADP and GDP. Are these...Ch. 25 - Prob. 25.9PCh. 25 - 25-10 Which DNA and RNA bases contain a carbonyl...
Ch. 25 - Prob. 25.11PCh. 25 - Prob. 25.12PCh. 25 - Prob. 25.13PCh. 25 - Prob. 25.14PCh. 25 - Prob. 25.15PCh. 25 - Prob. 25.16PCh. 25 - Prob. 25.17PCh. 25 - 25-18 What type of bond connects the base to the...Ch. 25 - Prob. 25.19PCh. 25 - 25-20 What constitutes the backbone of DNA?Ch. 25 - Prob. 25.21PCh. 25 - 25-22 In DNA, which carbon atoms of...Ch. 25 - 25-23 The sequence Of a short DNA segment is...Ch. 25 - Prob. 25.24PCh. 25 - Prob. 25.25PCh. 25 - Prob. 25.26PCh. 25 - Prob. 25.27PCh. 25 - Prob. 25.28PCh. 25 - 25-29 What constitutes the superstructure of...Ch. 25 - 25-30 What is the primary structure of DNA?Ch. 25 - 25-31 What is the secondary structure of DNA?Ch. 25 - 25-32 What is the major groove of a DNA helix?Ch. 25 - 25-33 What are the higher-order structures of DNA...Ch. 25 - 25-34 Which type Of RNA has enzyme activity? Where...Ch. 25 - 25-35 Which has the longest chains: tRNA, mRNA, or...Ch. 25 - 25-36 Which type of RNA contains modified...Ch. 25 - 25-37 Which type Of RNA has a sequence exactly...Ch. 25 - 25-38 Where is rRNA located in the cell?Ch. 25 - Prob. 25.39PCh. 25 - Prob. 25.40PCh. 25 - Prob. 25.41PCh. 25 - Prob. 25.42PCh. 25 - Prob. 25.43PCh. 25 - 25-44 What is IncRNA and what does it do?Ch. 25 - Prob. 25.45PCh. 25 - Prob. 25.46PCh. 25 - Prob. 25.47PCh. 25 - Prob. 25.48PCh. 25 - Prob. 25.49PCh. 25 - 25-50 Define: (a) Intron (b) ExonCh. 25 - 25-51 Does mRNA also have introns and exons?...Ch. 25 - Prob. 25.52PCh. 25 - Prob. 25.53PCh. 25 - 25-54 Do all genes code for a protein? If not,...Ch. 25 - 25-55 A DNA molecule normally replicates itself...Ch. 25 - 25-56 Which functional groups on the bases form...Ch. 25 - Prob. 25.57PCh. 25 - 25-58 Draw the structures of cytosine and guanine...Ch. 25 - 25-59 How many different bases are present in a...Ch. 25 - Prob. 25.60PCh. 25 - 25-61 Why is replication called semiconservative?Ch. 25 - 25-62 How does the removal of some positive...Ch. 25 - 25-63 Write the chemical reaction for the...Ch. 25 - Prob. 25.64PCh. 25 - 25-65 What are helicases? What is their function?Ch. 25 - 25-66 Can dATP Serve as a source for a primer?Ch. 25 - Prob. 25.67PCh. 25 - Prob. 25.68PCh. 25 - 25-69 In which direction is the DNA molecule...Ch. 25 - Prob. 25.70PCh. 25 - Prob. 25.71PCh. 25 - Prob. 25.72PCh. 25 - 25-73 From the perspective of the chain being...Ch. 25 - Prob. 25.74PCh. 25 - Prob. 25.75PCh. 25 - 25-76 When cytosine is deaminated, uracil is...Ch. 25 - Prob. 25.77PCh. 25 - 25-78 What are AP sites? Which enzyme creates...Ch. 25 - Prob. 25.79PCh. 25 - Prob. 25.80PCh. 25 - Prob. 25.81PCh. 25 - Prob. 25.82PCh. 25 - Prob. 25.83PCh. 25 - Prob. 25.84PCh. 25 - Prob. 25.85PCh. 25 - Prob. 25.86PCh. 25 - Prob. 25.87PCh. 25 - 25-88 (Chemical Connections 25B) How does...Ch. 25 - Prob. 25.89PCh. 25 - Prob. 25.90PCh. 25 - Prob. 25.91PCh. 25 - Prob. 25.92PCh. 25 - Prob. 25.93PCh. 25 - 25-94 (Chemical Connection 25D) What would be some...Ch. 25 - Prob. 25.95PCh. 25 - (Chemical Connection 25D) Why might a person make...Ch. 25 - Prob. 25.97PCh. 25 - Prob. 25.98PCh. 25 - Prob. 25.99PCh. 25 - Prob. 25.100PCh. 25 - Prob. 25.101PCh. 25 - Prob. 25.102PCh. 25 - Prob. 25.103PCh. 25 - -104 Why is it important that a DNA molecule be...Ch. 25 - Prob. 25.105PCh. 25 - Prob. 25.106PCh. 25 - Prob. 25.107PCh. 25 - Prob. 25.108PCh. 25 - -109 In sheep DNA, the mol% of adenine (A) was...Ch. 25 - Prob. 25.110PCh. 25 - Prob. 25.111PCh. 25 - Prob. 25.112PCh. 25 - Prob. 25.113PCh. 25 - -114 How could life have evolved if DNA leads to...Ch. 25 - Prob. 25.115PCh. 25 - Prob. 25.116PCh. 25 - Prob. 25.117P
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- What type of RNA has the shortest lifetime in cells?arrow_forwardThe codon UAA stops protein synthesis. Why does the sequence UAA in the following stretch of mRNA not cause any problems?arrow_forwardSuppose that 30% of the nucleotides in a DNA molecule are deoxyguanosine 5'- monophosphate, and that during DNA replication the percentage amounts of available nucleotide bases are 20% A, 20% C. 30% G. and 30% T. Which base would be depleted first in the replication process?arrow_forward
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