Concept explainers
(a)
To determine: The location of appearance of the radioactive isotope in a matured mRNA, if a eukaryotic cell is incubated with 32P labelled ATP at α position.
Introduction: ATP is utilized for various purposes during transcription and RNA processing. It is also used in DNA helicase activity associated with TFIIH to promote the unwinding of DNA template at the transcriptional start site prior to initiation. Radiolabeling of ATPs during transcription helps to find out the specific
(b)
To determine: The location of appearance of the radioactive isotope in a matured mRNA, if a eukaryotic cell is incubated with 32P labelled ATP at β position.
Introduction: ATP is utilized for various purposes during transcription and RNA processing. It is also used in DNA helicase activity associated with TFIIH to promote the unwinding of DNA template at the transcriptional start site prior to initiation. Radiolabeling of ATPs during transcription helps to find out the specific nucleotide sequences.
(c)
To determine: The location of appearance of the radioactive isotope in a matured mRNA, if a eukaryotic cell is incubated with 32P labelled ATP at γ position.
Introduction: ATP is utilized for various purposes during transcription and RNA processing. It is also used in DNA helicase activity associated with TFIIH to promote the unwinding of DNA template at the transcriptional start site prior to initiation. Radiolabeling of ATPs during transcription helps to find out the specific nucleotide sequences.
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