The isoelectric point of histidine is 7.64. Toward which electrode does histidine migrate on paper electrophoresis at pH 7.0?

Q: In a solution at pH 7.0, a polymer of lysine( polylysine) is a random coil with no secondary…

A: Polylysine forms a random coil at low pH , alpha helix at a pH above 10.6 and gets transformed into…

Q: Define the following terms :(i) Sorption(ii) Tyndall effect(iii) Electrophoresis

A: Here given few terms and we are asked to define them.

Q: 9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B…

A: According to the given pKa value and the following structure, the order of elution for the four B…

Q: Look up the pKa values for trimethylamine, dimethylamine, methylamine, and ammonia. Predict the…

A: pKb tells about the basicity of any organic base. Higher the pKb value, lower would be the basicity.

Q: general mechanism of electron ionization

A: 1 Electron ionisation is a part of mass spectroscopy. 2. the key points here are Analyte…

Q: You have the following polypeptides at pH 7.25 and are attempting to separate them using an anion…

A: For an amino acid residue,    If pKa > pH, the ionizable group remains in its protonated state.…

Q: Are the number of spots shown on SDS-PAGE electrophoresis dependant on whether the protein is a…

A: It is to explain that whether the number of spots shown on SDS-PAGE electrophoresis is dependent on…

Q: Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment.

A: Buffer is a solution that tends to maintain pH of the solution when acid or base is added to it.…

Q: Explain the method of finding martensite fraction during SIM ?

A: Martensite is formed in carbon steels by rapid cooling of the austenite form of iron at such a high…

Q: If the biochemist used SDS-Polyacrylamide Gel Electrophoresis , predict the correct order of…

A: SDS-Page is used to separate a mixture of proteins having their mass in the range of 5 - 250 kDa.…

Q: When using ion-selective electrodes, to comensate for a complex or unknown matrix, the __________…

A: Methods used to determine the analyte concentration: a) Least-squares analysis: this method is used…

Q: Rank the amino acids used in the experiment in Part II in the order of increasing solubility in…

A: The solubility of amino acids, generally, depending upon the nature of the side chain and they are…

Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…

A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…

Q: Which of the following tetrapeptides will be retained on a cation-exchange column at pH 8.2? O HGTH…

A: Ion exchange chromatography separates ions and molecules based on their net overall surface charge.…

Q: Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric…

A: Isoelectric point (pI) is the pH value at which the positive charge equals the negative charge,…

Q: Why is protonated pyrimidine (pKa = 1.0) more acidic than protonated pyridine (pKa = 5.2)?

A: The structure of protonated Pyrimidine and protonated Pyridine is as shown below:

Q: During the experiment, a student calculated the R¡value for glutamic acid of 0.29 and 0.45 for…

A: Chromatography is defined as the laboratory technique for the separation of components of a mixture…

Q: The pNa of a mixture prepared by mixing 200 ml of 0.00063 M of Nacl and 300 ml of 0.00040 M in…

A: The value of pNa is calculated by finding the concentration of Na+ ion in the mixture.

Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…

A:

Q: me amino group = 9.04, and pKaR for the side chain - 12.48

A: Deprotection of groups depends upon the it's pKa value when pH > it's pKa then deprotection…

Q: In what order would histidine, serine, aspartate, and valine be eluted with a buffer of pH 4 from a…

A: The order in which the following would the amino acids will be eluted with the buffer of pH 4 from a…

Q: What are the basic medium, acid medium, and zwitterion formulas of phenylalanine?

A: Phenyl alanine is an amino acid. Amino acid exist in three form depending upon the pH of solution.…

Q: A protein with a pI of 7.5 will be purified using ion-exchange chromatography.  Which ion exchange…

A: b)  A resin with sulfonic acid group is the correct option.

Q: What is electrophoresis due to?

A: Interpretation: What is electrophoresis due to?

Q: What are the chief differences between the Ninhydrin and Bradford assays for amino acid…

A: Ninhydrin and Bradford assay both are performed to determine amino acids. Nin hydrin reacts with…

Q: What is the fraction of EDTA at pH 10.7?

A: The concentration of hydrogen ion in the solution at the 10.7 pH is determined as follows.

Q: Which reaction is carried out in an acidic medium? Which reaction involves a reductant as an…

A: Since you have asked multiple question as per our company guidelines we are supposed to answer the…

Q: Calculate the Ksp for uraninite (reaction 1) and rutherfordine (reaction 2).             UO2 + 4 H+…

A: Solubility balance is a kind of complex balance that occurs when a solid chemicals compound is…

Q: Explain the following terms: (i) Electrophoresis (ii) Dialysis (iii) Tyndall effect

A: Since you have posted multiple independent questions in the same request, we will solve the first…

Q: The pka of ascorbic acid is 4.2 at 24C. At what pH is the ratio of the deprotonated form to the…

A: Henderson Hasselbach equation is pH=pKa+log[deprotonated form][protonated form]

Q: ind the isoelectric and zwitter ion of lysine, show the steps

A: Given the isoelectric and zwitter ion of lysine

Q: The buffer needed for a capillary electrophoresis experiment must have a pH of 10.0. Which weak acid…

A: Given,  pH = 10.0  Which weak acid is the best choice for the buffer? Assume ionic strength is…

Q: The isoelectric point, pI, of the protein hexokinase P-I is 5.25, while that of alkaline phosphatase…

A:

Q: What is the effect of temperature on chemisorption?

A: The process that deals with the accumulation of gas-solid or liquid, or gas molecules on another…

Q: d. Suppose you mistakenly acidify the filtrate with HCI instead of with HN03. What would you observe…

A: The halogen present in the organic compound is identified by following the procedure written below.…

Q: The isoelectric point of tyrosine is 5.63. Toward which electrode does tyrosine migrate on paper…

A: Amino acid are the substances which have an amine group, a carboxyl group and a side chain which is…

Q: A mixture of proteins (pepsinogen, myoglobin, and cytochrome c) is subjected to anion exchange…

A: a. Anion exchange resin has positive charge. Which attracts negative charged biomolecules. b. As…

Q: The following are results of analyses done using the vanadomolybdate spectrophotometric technique.…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Would the continuous assay of Alkaline phosphatase (kinetics lab) with PNPP as a substrate work if…

A: The macromolecular biological substances by which chemical reaction is accelerated is termed as an…

Q: The isoelectric point, pl, of the protein nitrate reductase is 4.2 , while that of superoxide…

A: We’ll answer the first part since the exact one wasn’t specified. Please submit a new question…

Q: Write a Chen ups reaction of EDTA binding with a heavy metal such as iron

A: EDTA is most wildely accepting chelating agent. EDTA has 2 nitrogen, 4 oxygen atom,that can form…

Q: 8. What properties cause the separation of amino acids in paper chromatography?

A: Different amino  acids have different partition coefficient between water and organic solvent.…

Q: The isoelectric point, pl, of the protein soybean trypsin inhibitor is 4.5 , while that of…

A: In proteins, the isoelectric point (pI) is defined as the pH at which a protein carries no net…

Q: If you had a mixture of proteins with different sizes, shapes, and charged and you separated them…

A: Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are…

Q: Studies on cAMP actions in cultured cells usually involve adding to the cell culture not cAMP, but…

A: Cyclic adenosine monophosphate (CAMP) is a secondary messenger molecule that plays an important role…