Brock Biology of Microorganisms (15th Edition)
Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 28, Problem 1AQ

Define the procedures you would use to isolate and identify a new pathogen. Keep in mind Koch’s postulates (Chapter 28, Problem 1AQ, Define the procedures you would use to isolate and identify a new pathogen. Keep in mind Kochs Figure 1.29) as you form your answer. Be sure to include growth-dependent assays, immunoassays, and molecular assays. Which of your assays could be adapted to be used as a routine, high-throughput test for rapid clinical diagnosis?

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Summary Introduction

To explain:

The procedure used to isolate and identify a new pathogen and the method would be more suitable to the high throughput test for rapid clinical diagnosis with perspective to growth dependent assays, immunoassays and molecular assays

Introduction:

Robert Koch a German microbiologist and physician is the father of modern microbiology. He made several achievements which include identification of key agents in tuberculosis, anthrax and cholera disease types. His postulates led a way to determine and identification of microorganisms based on morphology and key characteristics. He was awarded the Nobel Prize in 1905.

Explanation of Solution

A procedure used to isolate and identify the new pathogen is done by direct observation and culture method. Moreover, the pathogen is observed through direct observation of sputum and urethral exudates in infectious diseases. They include acid-fast stained Mycobacterium tuberculosis and Neisseria gonorrhea. Most of the pathogens are grown in cultures in the laboratory. In contrast, clinically important bacteria are grown and isolated in growth media.

Clinical diagnosis of growth dependent assays is used to report the findings. Depending upon the growth on enriched media, the pathogen is sub-cultured on media, which is designed to measure biochemical reactions. The media used can be differential or selective.

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Students have asked these similar questions
the following are the steps, listed in random order, for utilizing koch's postulates. what should be the second step out of the four listed? a. a microorganism is found to be present in every case of a disease. b. the same microbe discovered previously is isolated from the purposely infected host c. the pathogen is isolated and grown in pure culture d. the same disease results after isolated organism is inoculated into healthy host
You have learned many different techniques so far this semester that allow us to identify pathogenic microbes in the clinical setting. Why do you think rapid methods are so helpful? Why are traditional tests such as metabolic tests not always used to ID clinically important microbes?
Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.

Chapter 28 Solutions

Brock Biology of Microorganisms (15th Edition)

Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
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