MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Chapter 3, Problem 4R
Summary Introduction
Introduction:
A compound light microscope uses a light source and two pair of lens system for magnification. The maximum magnification of compound light microscope is 1000X, under oil immersion.
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Chapter 3 Solutions
MICROBIOLOGY-ACCESS >CUSTOM<
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. At 100X you are unable to see the diatom. Explain why. Explain what correction (s) should be made to allow you to see the diatom using the 100X lens. After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.arrow_forwardPlease explain the tablearrow_forwardDescribe how the images obtained using a confocal microscope would appear different to those obtained using a widefield microscope.arrow_forward
- If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400xarrow_forwardIf the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUarrow_forwardWould phase microscopy be of any particular value when examining a stained specimen? Explain.arrow_forward
- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forward3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?arrow_forwardWhat are the differences and similarities between Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Please explain in detail.arrow_forward
- Staining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?arrow_forwardBased on the video (link provided below: https://youtu.be/SUo2fHZaZCU) give 10 steps on handling the microscopearrow_forwardWhat’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?arrow_forward
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