Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 30, Problem 13P
Interpretation Introduction

Interpretation:

The Shine-Dalgarno sequence and its role in the variation of the efficiency of initiation of the protein synthesis needs to be defined.

Concept Introduction:

Australian scientists Lynn Dalgarno and John Shine proposed the Shine-Dalgarno sequence. Usually situated 8 bases upstream of the initial codon AUG, it is the ribosomal binding site within the mRNA. This sequence is present both within archaea, as well as in bacteria, and exists in some mitochondrial and chloroplastic transcripts. The sequence of RNA supports recruiting the ribosome towards the mRNA to start protein synthesis via aligning the ribosome with the initial codon.

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The base sequence of the gene coding for a short polypeptide is 5’CTACGCTAGGCGATTGATCATC’3.  What would be the base sequence of the mRNA transcribed from this gene? Highlight the start codon sequence    State the amino acid sequence of the polypeptide translated from this mRNA    Based on the information from part (a) and (b), describe the process used by eukaryotes to produce protein.
Template strand of DNA is: 3’ TACATAACCGGGCCCATATCGGCCATTTGC5’. 2a). Following transcription, what is the total number of codons in the mRNA transcript? 2 b). Where is the start codon located in this mRNA transcript? 2c). Following translation of this mRNA transcript, how many amino acids will the proteincontain and identify the amino acids sequence of this gene from a genetic code table*.*Note= using a genetic code table
The 3 major forms of RNA (mRNA, tRNA, & rRNA) interact during translation. c)Compared to the average stability of mRNA in E.coli, is mRNA in a typical human cell more stable or less stable? Why?
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