(a) Interpretation: The equation for the calibration curve, standard deviations of the slope, intercept from the data of doxorubicin by LIF, its peak value and the R 2 value needs to be determined. Concept introduction: The standard deviation is to tell how the measurements of group are spread out from average. In other words, it defines how far a set of numbers lie apart. If the value obtained is low, it means that most of the numbers are close to average, but if the value obtained is high, it means the number are spread out.

BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213
BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

Solutions

Chapter 30, Problem 30.12QAP
Interpretation Introduction

(a)

Interpretation:

The equation for the calibration curve, standard deviations of the slope, intercept from the data of doxorubicin by LIF, its peak value and the R2 value needs to be determined.

Concept introduction:

The standard deviation is to tell how the measurements of group are spread out from average. In other words, it defines how far a set of numbers lie apart. If the value obtained is low, it means that most of the numbers are close to average, but if the value obtained is high, it means the number are spread out.

Interpretation Introduction

(b)

Interpretation:

The equation should be rearranged, and concentration should be expressed in terms of measured area.

Concept introduction:

For a straight-line curve, the equation is represented as follows:

y=mx+c

Here, m is slope of the graph and c is intercept of the graph.

Interpretation Introduction

(c)

Interpretation:

The LOD in terms of moles needs to be determined, if the LOD for DOX is 3x1011 M keeping the injection volume as 100 pL.

Concept introduction:

Laser induced fluorescence is spectroscopic method in which a molecule is excited to higher energy level by absorbing light. It helps to study the structure of molecule.

Interpretation Introduction

(d)

Interpretation:

The concentrations and standard deviations of the unknown DOX injected needs to be determined, if the peaks of two DOX unknown samples injected is 11.3 and 6.97.

Concept introduction:

The laser induced fluorescence is method wherein atoms or molecules is excited by passing laser light that excites them to higher energy levels releasing light spontaneously. It is useful in studying the structure of molecules.

Interpretation Introduction

(e)

Interpretation:

The time required for applied voltage to be doubled needs to be determined, if the DOX concentration is 300s. Also, the time needs to be determined, if the capillary length is doubled.

Concept introduction:

The laser induced fluorescence is method wherein atoms or molecules is excited though passing a laser light that excites them to higher energy levels releasing light spontaneously. It is useful in studying the structure of molecules.

Interpretation Introduction

(f)

Interpretation:

The value of N if the capillary length is doubled at the same voltage and the value of N if the applied voltage is doubled with same capillary length.

Concept introduction:

The laser induced fluorescence involves excitation of molecules or atoms to higher energy levels releasing light spontaneously through passing of laser light. It is useful in studying the structure of molecules.

Interpretation Introduction

(g)

Interpretation:

The plate height for capillary with N=100,000 for 40.6cm long capillary having diameter of 50µm needs to be determined.

Concept introduction:

The laser induced fluorescence involves excitation of molecules or atoms to higher energy levels releasing light spontaneously through passing of laser light. It is useful in studying the structure of molecules.

Interpretation Introduction

(h)

Interpretation:

The value of variance σ2 needs to be determined, if the plate height for capillary with N=100,000 is 40.6cm having diameter of 50µm.

Concept introduction:

The laser induced fluorescence involves excitation of molecules or atoms to higher energy levels releasing light spontaneously through passing a laser light. It is useful in studying the structure of molecules.

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