The determination of streptomycin in 500g of broth is done by the mixing of pure antibiotic, 14 C of 12.5mg. For the 30 min count the specific activity of this preparation is 240cpm/mg. The purified streptomycin of amount 0.112 mg is isolated from the mixture and at this condition the number of count is 675 for 60.0min. The concentration in ppm streptomycin is to be determined. Concept introduction: The amount of streptomycin is calculated by the isotope dilution method which is given as- m x = R t R m × m m − m t ……………………….. (1) Where, m x = mass of inactive sample R t = counting rate R m = counting rate of inactive analyte m m = isolated mass which is purified from the mixture of radioactive tracer m t = mass of radioactive tracer a c t i v i t y o f t r a c e r = S p e c i f i c a c t i v i t y × A m o u n t o f t r a c e r

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Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213
BuyFind

Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

Solutions

Chapter 32, Problem 32.18QAP
Interpretation Introduction

Interpretation:

The determination of streptomycin in 500g of broth is done by the mixing of pure antibiotic, 14C of 12.5mg. For the 30 min count the specific activity of this preparation is 240cpm/mg. The purified streptomycin of amount 0.112 mg is isolated from the mixture and at this condition the number of count is 675 for 60.0min. The concentration in ppm streptomycin is to be determined.

Concept introduction:

The amount of streptomycin is calculated by the isotope dilution method which is given as-

mx=RtRm×mmmt ……………………….. (1)

Where,

mx = mass of inactive sample

Rt = counting rate

Rm = counting rate of inactive analyte

mm = isolated mass which is purified from the mixture of radioactive tracer

mt = mass of radioactive tracer

activity of tracer= Specific activity ×Amount of tracer

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