The determination of streptomycin in 500g of broth is done by the mixing of pure antibiotic, of 12.5mg. For the 30 min count the specific activity of this preparation is 240cpm/mg. The purified streptomycin of amount 0.112 mg is isolated from the mixture and at this condition the number of count is 675 for 60.0min. The concentration in ppm streptomycin is to be determined.
The amount of streptomycin is calculated by the isotope dilution method which is given as-
= mass of inactive sample
= counting rate
= counting rate of inactive analyte
= isolated mass which is purified from the mixture of radioactive tracer
= mass of radioactive tracer
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