Science

ChemistryPrinciples of Instrumental AnalysisThe determination of streptomycin in 500g of broth is done by the mixing of pure antibiotic, 14 C of 12.5mg. For the 30 min count the specific activity of this preparation is 240cpm/mg. The purified streptomycin of amount 0.112 mg is isolated from the mixture and at this condition the number of count is 675 for 60.0min. The concentration in ppm streptomycin is to be determined. Concept introduction: The amount of streptomycin is calculated by the isotope dilution method which is given as- m x = R t R m × m m − m t ……………………….. (1) Where, m x = mass of inactive sample R t = counting rate R m = counting rate of inactive analyte m m = isolated mass which is purified from the mixture of radioactive tracer m t = mass of radioactive tracer a c t i v i t y o f t r a c e r = S p e c i f i c a c t i v i t y × A m o u n t o f t r a c e rStart your trial now! First week only $4.99!*arrow_forward*

BuyFind*launch*

7th Edition

Douglas A. Skoog + 2 others

Publisher: Cengage Learning

ISBN: 9781305577213

Chapter 32, Problem 32.18QAP

Interpretation Introduction

**Interpretation:**

The determination of streptomycin in 500g of broth is done by the mixing of pure antibiotic,

**Concept introduction:**

The amount of streptomycin is calculated by the isotope dilution method which is given as-

Where,