Chapter 5, Problem 1RE

RECALL What types of homogenization techniques are available for solubilizing a protein?

Interpretation Introduction

Interpretation:

The homogenization techniques that are available for solubilizing a protein are to be named.

Concept introduction:

Homogenization is a process in which proteins gets released from the cell and subcellular organelles.

To isolate a protein of interest from the cell, it is important to break open the cells and then solubilize the protein in a liquid. This can be archived by various techniques.

Answer to Problem 1RE

A Potter–Elvejhem homogenizer technique, a sonication or using a blender.

Explanation of Solution

Isolated cells and tissues are mixed with a buffer solution (the solution that keeps pH of the system intact) and put into a blender. Grinding the tissue releases the soluble protein. A disadvantage of this process is that it will break all the cell organelles including mitochondria, lysosome, ribosome, and makes the purification process difficult.

In Potter–Elvehjem homogenizer technique, the cells and tissues are put into a thick-walled test tube and a tight-fitting plunger is used to break open the cells by squeezing. This process creates a homogenate (liquid containing cell contents and fragments) while keeping many cell organelles intact.

In the sonicator technique, sound waves are used to break open the cells.

Conclusion

The homogenization techniques that are available for solubilizing a protein are sonication, using a blender and Potter–Elvejhem homogenizer technique.