Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 5, Problem 3P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Solving the Sequence of an Oligopeptide From Sequence Analysis Data Amino acid analysis of a decapeptide revealed the presence of the following products:

Chapter 5, Problem 3P, Answers to all problems are at the end of this book. Detailed solutions are available in the Student , example  1

The following facts were observed:

  1. Neither car boxy peptidase A nor B treatment of the- decapeptide had any effect.
  2. Trypsin treatment yielded two tetrapcptides and free Lys.
  3. Clostripain treatment yielded a tetrapcptide and a hexapeptidc.
  4. Cyanogen bromide treatment yielded an octapeptide and a dipeptide of sequence NP (using the one-letter codes).
  5. Chymotrypsin treatment yielded two tripeptides and a telrapeptide. The N-terminal chymotryptic peptide had a net charge of — 1 at neutral pi I and a net charge of —3 al pH 12.
  6. One cycle of Ed man degradation gave the PTH derivative

Chapter 5, Problem 3P, Answers to all problems are at the end of this book. Detailed solutions are available in the Student , example  2

What is the ammo acid sequence of this decapeptide?

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