Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Question
Chapter 5, Problem 5.12QAP
Interpretation Introduction
Interpretation:
The improvement in S/N in progressing from spectrum A to spectrum D in figure 5-15 needs to be calculated.
Concept introduction:
Signal-to-noise ratio (S/N) is a scale used in engineering and science to compare the level of the signal to the background noise. It is defined as ratio of power of signal to the noise and it is expressed in decibels.
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fill in the column thats empty by finding the standard deviation for each average absorbance, with work please.
Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L).
Data for YELLOW: peak absorbance used on spectrophotometer =420nm
Cuvette 1 - 100% dye = 0.680 absorption
Cuvette 2 - 75% dye = 0.510 absorption
Cuvette 3 - 50% dye = 0.340 absorption
Cuvette 4 - 25% dye = 0.170 absorption
Data for RED - peak absorption used on spectrophotometer = 500nm
Cuvette 1 - 100% dye: 0.621 absorption
Cuvette 2 - 75%: 0.466 absorption
Cuvette 3 - 50% dye: 0.311 absorption
Cuvette 4 - 25% dye: 0.1555 absorption
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ISBN:9781305577213
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Publisher:Cengage Learning