Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 5, Problem 6P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
Solving the Sequence of an Oligopeptide From Sequence Analysis Data Amino acid analysis of ill octapeptide gave the following results:
1 Ala 1 Arg 1 Asp 1 gly 3 he 1 Val 1 NH4+
The following facts were observed:
a. Trypsin treatment yielded a pentapeptide and a tripeptide.
b. Chemical reduction of the free or-COOH and subsequent acid hydrolysis yielded 2-aminopropanol.
c. Partial aeid hydrolysis of the tryptic pentapeptide yielded, among other products, two dipeptides, each of which, contained C-terminal isoleucine. One of these dipeptides migrated as an anionic species upon electrophoresis at neutral pH.
What is an amino acid sequence of the octapeptide? Four sequences art possible, but only one suits the authors. Why?
The tryptic tripeplide was degraded in an Edman sequenator, yielding first A, then B:
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Chapter 5 Solutions
Biochemistry
Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...Ch. 5 - Answers to all problems are at the end of this...
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