Science
BiochemistryBiochemistryREFLECT AND APPLY You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate precipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.
REFLECT AND APPLY You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate precipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.
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Biochemistry
9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135
BuyFindarrow_forward
Biochemistry
9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135
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Chapter
Section
Chapter 5, Problem 9RE
Textbook Problem
REFLECT AND APPLY You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate precipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.
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Chapter 5 Solutions
Biochemistry
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addCh. 5 - RECALL What types of homogenization techniques are...Ch. 5 - RECALL When would you choose to use a...Ch. 5 - RECALL What is meant by salting out? How does it...Ch. 5 - RECALL What differences between proteins are...Ch. 5 - RECALL How could you isolate mitochondria from...Ch. 5 - RECALL Can you separate mitochondria from...Ch. 5 - RECALL Give an example of a scenario in which you...Ch. 5 - RECALL Describe a procedure for isolating a...Ch. 5 - REFLECT AND APPLY You are purifying a protein for...Ch. 5 - REFLECT AND APPLY If you had a protein X, which is...
Ch. 5 - RECALL What is the basis for the separation of...Ch. 5 - RECALL What is the order of elution of proteins on...Ch. 5 - RECALL What are two ways that a compound can be...Ch. 5 - RECALL What are two ways that a compound can be...Ch. 5 - RECALL Why do most people elute bound proteins...Ch. 5 - RECALL What are two types of compounds that make...Ch. 5 - RECALL Draw an example of a compound that would...Ch. 5 - RECALL How can gel-filtration chromatography be...Ch. 5 - REFLECT AND APPLY Sephadex G-75 has an exclusion...Ch. 5 - REFLECT AND APPLY Referring to Question 19, could...Ch. 5 - RECALL What is the main difference between reverse...Ch. 5 - RECALL How does HPLC differ from ion-exchange...Ch. 5 - REFLECT AND APPLY Design an experiment to purify...Ch. 5 - REFLECT AND APPLY Referring to Question 23, how...Ch. 5 - REFLECT AND APPLY What could be an advantage of...Ch. 5 - REFLECT AND APPLY You wish to separate and purify...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY In reverse-phase HPLC, the...Ch. 5 - REFLECT AND APPLY Gel-filtration chromatography is...Ch. 5 - RECALL What physical parameters of a protein...Ch. 5 - RECALL What types of compounds make up the gels...Ch. 5 - RECALL Of the two principal polymers used in...Ch. 5 - RECALL What types of macromolecules are usually...Ch. 5 - RECALL If you had a mixture of proteins with...Ch. 5 - RECALL What does SDSPAGE stand for? What is the...Ch. 5 - RECALL How does the addition of sodium...Ch. 5 - RECALL Why is the order of separation based on...Ch. 5 - RECALL The accompanying figure is from an...Ch. 5 - RECALL Why is it no longer considered necessary to...Ch. 5 - RECALL What useful information might you get if...Ch. 5 - REFLECT AND APPLY Show by a series of equations...Ch. 5 - REFLECT AND APPLY Why can the Edman degradation...Ch. 5 - REFLECT AND APPLY What would happen during an...Ch. 5 - REFLECT AND APPLY A sample of an unknown peptide...Ch. 5 - REFLECT AND APPLY A sample of a peptide of unknown...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - REFLECT AND APPLY Amino acid compositions can be...Ch. 5 - REFLECT AND APPLY Assume that you are getting...Ch. 5 - REFLECT AND APPLY Which enzymes or chemicals would...Ch. 5 - REFLECT AND APPLY With which amino acid sequences...Ch. 5 - BIOCHEMICAL CONNECTIONS What are the two principal...Ch. 5 - BIOCHEMICAL CONNECTIONS What is the advantage of...Ch. 5 - RECALL What is the basis for the technique called...Ch. 5 - RECALL What is the difference between a primary...Ch. 5 - RECALL What are some ways that the...Ch. 5 - RECALL What are the main procedures involved in a...Ch. 5 - RECALL Where did western blot get its name?Ch. 5 - REFLECT AND APPLY What is an advantage of using an...Ch. 5 - REFLECT AND APPLY What is the advantage to...Ch. 5 - REFLECT AND APPLY What is the point to labeling a...Ch. 5 - RECALL What is proteomics?Ch. 5 - REFLECT AND APPLY What is the purpose of the tag...Ch. 5 - REFLECT AND APPLY What are some of the assumptions...
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