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BiochemistryBiochemistry9th Edition
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REFLECT AND APPLY Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not?

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Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135

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Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135
Chapter 6, Problem 15RE
Textbook Problem
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REFLECT AND APPLY Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not?

Interpretation Introduction

Interpretation:

There is an experimental method whose rate of reaction can be determined with the help of a spectrophotometer. Whether the pH meter can be used to monitor the progress of the reaction or not is to be explained.

Concept introduction:

The pH meter is an instrument, which measures the hydrogen ion activity in the given solution. It indicates the acidity and alkalinity of the solution. The pH meter is used in various types of applications that range from laboratory experiments to the environmental pollution control.

The chemical reactions, in which the enzymes are involved, run in buffer solution so as to maintain the defined range of pH according to the enzyme.

Explanation of Solution

Given information:

The enzyme lactate dehydrogenase catalyzes the reaction in which pyruvate is converted into lactate and NADH into NAD+. NADH absorbs light at 340 nm in the near ultraviolet region of the electromagnetic spectrum, but NAD+ does not.

Pyruvate + NADH + H+ → Lactate +

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