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Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135

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BuyFindarrow_forward

Biochemistry

9th Edition
Mary K. Campbell + 2 others
Publisher: Cengage Learning
ISBN: 9781305961135
Chapter 6, Problem 15RE
Textbook Problem
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REFLECT AND APPLY Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not?

Interpretation Introduction

Interpretation:

There is an experimental method whose rate of reaction can be determined with the help of a spectrophotometer. Whether the pH meter can be used to monitor the progress of the reaction or not is to be explained.

Concept introduction:

The pH meter is an instrument, which measures the hydrogen ion activity in the given solution. It indicates the acidity and alkalinity of the solution. The pH meter is used in various types of applications that range from laboratory experiments to the environmental pollution control.

The chemical reactions, in which the enzymes are involved, run in buffer solution so as to maintain the defined range of pH according to the enzyme.

Explanation of Solution

Given information:

The enzyme lactate dehydrogenase catalyzes the reaction in which pyruvate is converted into lactate and NADH into NAD+. NADH absorbs light at 340 nm in the near ultraviolet region of the electromagnetic spectrum, but NAD+ does not.

Pyruvate+NADH+H+Lactate+

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Chapter 6 Solutions

Biochemistry
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Ch. 6 - REFLECT AND APPLY An enzyme catalyzes the...Ch. 6 - REFLECT AND APPLY Can the presence of a catalyst...Ch. 6 - RECALL For the hypothetical reaction 3A+2B2C+3D...Ch. 6 - REFLECT AND APPLY The enzyme lactate dehydrogenase...Ch. 6 - REFLECT AND APPLY Would you use a pH meter to...Ch. 6 - REFLECT AND APPLY Suggest a reason for carrying...Ch. 6 - RECALL Distinguish between the lock-and-key and...Ch. 6 - RECALL Using an energy diagram, show why the...Ch. 6 - REFLECT AND APPLY Other things being equal, what...Ch. 6 - REFLECT AND APPLY Amino acids that are far apart...Ch. 6 - REFLECT AND APPLY If only a few of the amino acid...Ch. 6 - RECALL Show graphically how the reaction velocity...Ch. 6 - RECALL Define steady state, and comment on the...Ch. 6 - RECALL How is the turnover number of an enzyme...Ch. 6 - MATHEMATICAL For an enzyme that displays...Ch. 6 - MATHEMATICAL Determine the values of KM and Vmax...Ch. 6 - MATHEMATICAL The kinetic data in the following...Ch. 6 - MATHEMATICAL The enzyme -methylaspartase catalyzes...Ch. 6 - MATHEMATICAL The hydrolysis of a...Ch. 6 - MATHEMATICAL For the Vmax obtained in Question 26,...Ch. 6 - MATHEMATICAL You do an enzyme kinetic experiment...Ch. 6 - REFLECT AND APPLY The enzyme D-amino acid oxidase...Ch. 6 - REFLECT AND APPLY Why is it useful to plot rate...Ch. 6 - REFLECT AND APPLY Under what conditions can we...Ch. 6 - BIOCHEMICAL CONNECTIONS Why does acetazolamide...Ch. 6 - BIOCHEMICAL CONNECTIONS How did scientists...Ch. 6 - BIOCHEMICAL CONNECTIONS How do the KM values for...Ch. 6 - BIOCHEMICAL CONNECTIONS When does the kcat/KM...Ch. 6 - RECALL What are the three most common mechanisms...Ch. 6 - RECALL What is the biggest difference between a...Ch. 6 - RECALL How do scientists determine the KM of a...Ch. 6 - REFLECT AND APPLY If you graph the velocity of an...Ch. 6 - RECALL Show graphically the dependence of reaction...Ch. 6 - RECALL Do all enzymes display kinetics that obey...Ch. 6 - RECALL How can you recognize an enzyme that does...Ch. 6 - RECALL If we describe an enzyme like aspartate...Ch. 6 - RECALL How can competitive and pure noncompetitive...Ch. 6 - RECALL Why does a competitive inhibitor not change...Ch. 6 - RECALL Why does a pure noncompetitive inhibitor...Ch. 6 - RECALL Distinguish between the molecular...Ch. 6 - RECALL Can enzyme inhibition be reversed in all...Ch. 6 - RECALL Why is a Lineweaver-Burk plot useful in...Ch. 6 - RECALL Where do lines intersect on a...Ch. 6 - RECALL What is the difference between pure and...Ch. 6 - REFLECT AND APPLY Why can we say that having a...Ch. 6 - REFLECT AND APPLY When we compare the binding of I...Ch. 6 - RECALL Why does the apparent KM decrease in the...Ch. 6 - RECALL What is a suicide substrate? Why are they...Ch. 6 - RECALL If we made a Lineweaver-Burk plot of an...Ch. 6 - MATHEMATICAL Draw LineweaverBurk plots for the...Ch. 6 - MATHEMATICAL For the following aspartase reaction...Ch. 6 - REFLECT AND APPLY Is it good (or bad) that enzymes...Ch. 6 - REFLECT AND APPLY Noncompetitive inhibition is a...Ch. 6 - BIOCHEMICAL CONNECTIONS You have been hired by a...Ch. 6 - REFLECT AND APPLY Would you expect an irreversible...Ch. 6 - REFLECT AND APPLY Would you expect the structure...Ch. 6 - BIOCHEMICAL CONNECTIONS What part of the HIV...Ch. 6 - BIOCHEMICAL CONNECTIONS What part of the HIV...

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