What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

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Answer 1

Textbook Question

Chapter 6, Problem 1AQ

What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

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Students have asked these similar questions

Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in yeast (a eukaryote) in such a way that it is properly flanked by a promoter and a transcriptional terminator. The bacterial DNA is transcribed and the RNA correctly processed, but only the protein nearest the promoter is produced. Can you suggest why?

As discussed in the text, promoters were originally identified as consensus sequences upstream from transcriptional start sites. What additional evidence might support the assignment of these sequences as parts of promoters?

In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answer

Answer 2

Textbook Question

Chapter 6, Problem 1AQ

What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Textbook Question

Chapter 6, Problem 1AQ

What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 6, Problem 1AQ

What would happen to regulation from a promoter under negative control if the region where the regulatory protein binds was deleted? What If the promoter was under positive control? Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E., coll. Why?

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

Answer 8

Expert Solution

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Answer 9

Expert Solution

Answer 10

Expert Solution

Answer 11

Expert Solution

Answer 12

Summary Introduction

To explain:

What would happen to regulation process from a promoter under negative control if the region where the regulatory protein binds was deleted. What if the promoter was under positive control.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Answer 13

Explanation of Solution

The operonal gene products are produced in high amount when the regulatory protein-binding region of a promoter are deleted under negative control. Because of the repressor protein cannot bind to the promoter region. The operonal gene products are not produced when the regulatory protein-binding region of a promoter are deleted. This is because of the activator which cannot attach itself to the promoter region.

Answer 14

Expert Solution

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

Answer 15

Expert Solution

Answer 16

Expert Solution

Answer 17

Expert Solution

Answer 18

Summary Introduction

To explain:

Promoters from Escherichia coli under positive control are not close matches to the promoter consensus sequence for E. coli. Why.

Concept introduction:

The regulation of transcription is effective in negative control, because the cell needs the signal for the presence of enough biosynthetic products for their growth. The transcription is also controlled by both negatively or positively because of the presence of lactose and absence of glucose in the culture medium.

Answer 19

Explanation of Solution

The DNA consequences matches closely with the promoter region that leads to the binding of the RNA polymerase to the promoter region and stimulates the transcription without the presence of other factors. Thus, a quite different transcription control is seen in the promoters under positive control.

Answer 20

Ch. 6.1 - What is protein domain?Ch. 6.1 - Why are most DMA-binding proteins specific to...Ch. 6.1 - Describe why a protein that binds to a specific...Ch. 6.2 - Why is negative control so named?Ch. 6.2 - How does a repressor inhibit the synthesis of a...Ch. 6.2 - Induction is considered the opposite of enzyme...Ch. 6.3 - Prob. 1MQ Ch. 6.3 - Prob. 2MQ Ch. 6.3 - What is the difference between an operon and a...Ch. 6.4 - Prob. 1MQ

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